Robust 3D reconstruction and identification of dendritic spines from optical microscopy imaging

Firdaus Janoos, Kishore Mosaliganti, Xiaoyin Xu, Raghu Machiraju, Kun Huang, Stephen T.C. Wong

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

In neurobiology, the 3D reconstruction of neurons followed by the identification of dendritic spines is essential for studying neuronal morphology, function and biophysical properties. Most existing methods suffer from problems of low reliability, poor accuracy and require much user interaction. In this paper, we present a method to reconstruct dendrites using a surface representation of the neuron. The skeleton of the dendrite is extracted by a procedure based on the medial geodesic function that is robust and topology preserving, and it is used to accurately identify spines. The sensitivity of the algorithm on the various parameters is explored in detail and the method is shown to be robust.

Original languageEnglish (US)
Pages (from-to)167-179
Number of pages13
JournalMedical Image Analysis
Volume13
Issue number1
DOIs
StatePublished - Feb 2009

Keywords

  • 3D reconstruction
  • Curve-skeleton
  • Dendrite
  • Medial geodesic function
  • Neuron
  • Spine

ASJC Scopus subject areas

  • Computer Graphics and Computer-Aided Design
  • Computer Vision and Pattern Recognition
  • Radiology Nuclear Medicine and imaging
  • Health Informatics
  • Radiological and Ultrasound Technology

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