RNase mitochondrial RNA processing correctly cleaves a novel R loop at the mitochondrial DNA leading-strand origin of replication

Daniel Y. Lee, David A. Clayton

Research output: Contribution to journalArticle

59 Scopus citations

Abstract

The precursor primer RNA for mammalian mitochondrial DNA leading-strand replication remains as a persistent R loop formed during transcription through the mitochondrial DNA control region. We have examined model R loops, which exist in a novel and physiologically accurate preprimer conformation, as potential substrates for mammalian RNase mitochondrial RNA processing (MRP). Mouse RNase MRP accurately cleaves an R loop containing the mouse mitochondrial DNA origin. The multiple cleavage sites on the R-loop substrate match the priming sites observed in vivo, suggesting that RNase MRP alone is capable of generating virtually all of the leading-strand replication primers.

Original languageEnglish (US)
Pages (from-to)582-592
Number of pages11
JournalGenes and Development
Volume11
Issue number5
DOIs
StatePublished - Mar 1 1997

Keywords

  • mitochondrial RNA processing
  • R loop
  • Replication priming
  • RNA-DNA hybrid
  • RNase MRP

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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