Riboflavin-enhanced transport of serum albumin across the distal pulmonary epithelium

O. Douglas Wangensteen, Marilyn M. Bartlett, Jonathan K. James, Zhen Fan Yang, Philip S. Low

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Purpose. Conjugation of bovine serum albumin (BSA) with riboflavin (BSA-riboflavin) increases its uptake into cultured epithelial cells. Our purpose was to determine whether transport of BSA-riboflavin across the intact distal pulmonary epithelium is also increased, and whether transcytosis plays a role. Methods. In anesthetized rats, we instilled 3H-BSA-riboflavin or 3H-BSA into the trachea and measured their appearance in blood. In isolated, perfused rat lungs we measured the distal pulmonary epithe lium permeability-surface area product (PS) for FITC-BSA or FITC-BSA-riboflavin. Results. In intact rats we found 2.1 times more 3H-BSA-riboflavin than 3H-BSA appeared in blood 60 min after intratracheal instillation of the protein. In isolated, perfused rat lungs we found that BSA-riboflavin had double the PS of BSA (2.63 vs. 1.46 x 10-5 cm3/sec). The addition of transcytosis inhibitors monensin or nocodazole (both 3 x 10-5 M) reduced the BSA-riboflavin PS to that of BSA and had no effect on the PS of unconjugated BSA. Simultaneous measurements of 3H-sucrose PS showed no differences in paracellular transport among any of the experimental groups. Conclusions. Conjugation with riboflavin increases the flux of BSA across the distal pulmonary epithelium. The increased transport appears to be due to transcytosis, which apparently does not play a significant role in the movement of unconjugated BSA across the distal pulmonary epithelium.

Original languageEnglish (US)
Pages (from-to)1861-1864
Number of pages4
JournalPharmaceutical Research
Volume13
Issue number12
DOIs
StatePublished - Dec 1 1996

Keywords

  • Paracellular tranport
  • Permeability
  • Pulmonary drug delivery
  • Sucrose
  • Transcytosis

ASJC Scopus subject areas

  • Chemistry(all)
  • Pharmaceutical Science
  • Pharmacology

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