Revisiting the conformation and dynamics of DNA in slitlike confinement

Jing Tang; Stephen L. Levy; Daniel W. Trahan; Jeremy J. Jones; Harold G. Craighead; Patrick S. Doyle

doi:10.1021/ma101157x

Revisiting the conformation and dynamics of DNA in slitlike confinement

Jing Tang, Stephen L. Levy, Daniel W. Trahan, Jeremy J. Jones, Harold G. Craighead, Patrick S. Doyle

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111 Scopus citations

Abstract

We experimentally investigated the equilibrium conformation and dynamics of single DNA molecules in slitlike nanochannels. We measured the in-plane radius of gyration (R_II), diffusivity (D), and longest relaxation time (t) of ?-DNA (48.5 kbp) as functions of the slit height using fluorescence microscopy. Our results show that the in-plane radius of gyration increases monotonically with decreasing slit height, in contrast to results from Bonthuis et al.(15)but in agreement with our simulations and those of other groups. In strong confinement (slit height <100 nm), the scaling of D, t, and R _II with slit height does not show an evident change, suggesting that the transition from the de Gennes regime to the Odijk regime is gradual and broad.

Original language	English (US)
Pages (from-to)	7368-7377
Number of pages	10
Journal	Macromolecules
Volume	43
Issue number	17
DOIs	https://doi.org/10.1021/ma101157x
State	Published - Sep 14 2010

ASJC Scopus subject areas

Organic Chemistry
Polymers and Plastics
Inorganic Chemistry
Materials Chemistry

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title = "Revisiting the conformation and dynamics of DNA in slitlike confinement",

abstract = "We experimentally investigated the equilibrium conformation and dynamics of single DNA molecules in slitlike nanochannels. We measured the in-plane radius of gyration (RII), diffusivity (D), and longest relaxation time (t) of ?-DNA (48.5 kbp) as functions of the slit height using fluorescence microscopy. Our results show that the in-plane radius of gyration increases monotonically with decreasing slit height, in contrast to results from Bonthuis et al.(15)but in agreement with our simulations and those of other groups. In strong confinement (slit height <100 nm), the scaling of D, t, and R II with slit height does not show an evident change, suggesting that the transition from the de Gennes regime to the Odijk regime is gradual and broad.",

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T1 - Revisiting the conformation and dynamics of DNA in slitlike confinement

AU - Tang, Jing

AU - Levy, Stephen L.

AU - Trahan, Daniel W.

AU - Jones, Jeremy J.

AU - Craighead, Harold G.

AU - Doyle, Patrick S.

PY - 2010/9/14

Y1 - 2010/9/14

N2 - We experimentally investigated the equilibrium conformation and dynamics of single DNA molecules in slitlike nanochannels. We measured the in-plane radius of gyration (RII), diffusivity (D), and longest relaxation time (t) of ?-DNA (48.5 kbp) as functions of the slit height using fluorescence microscopy. Our results show that the in-plane radius of gyration increases monotonically with decreasing slit height, in contrast to results from Bonthuis et al.(15)but in agreement with our simulations and those of other groups. In strong confinement (slit height <100 nm), the scaling of D, t, and R II with slit height does not show an evident change, suggesting that the transition from the de Gennes regime to the Odijk regime is gradual and broad.

AB - We experimentally investigated the equilibrium conformation and dynamics of single DNA molecules in slitlike nanochannels. We measured the in-plane radius of gyration (RII), diffusivity (D), and longest relaxation time (t) of ?-DNA (48.5 kbp) as functions of the slit height using fluorescence microscopy. Our results show that the in-plane radius of gyration increases monotonically with decreasing slit height, in contrast to results from Bonthuis et al.(15)but in agreement with our simulations and those of other groups. In strong confinement (slit height <100 nm), the scaling of D, t, and R II with slit height does not show an evident change, suggesting that the transition from the de Gennes regime to the Odijk regime is gradual and broad.

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Revisiting the conformation and dynamics of DNA in slitlike confinement

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