Revisiting the conformation and dynamics of DNA in slitlike confinement

Jing Tang, Stephen L. Levy, Daniel W. Trahan, Jeremy J. Jones, Harold G. Craighead, Patrick S. Doyle

Research output: Contribution to journalArticlepeer-review

111 Scopus citations


We experimentally investigated the equilibrium conformation and dynamics of single DNA molecules in slitlike nanochannels. We measured the in-plane radius of gyration (RII), diffusivity (D), and longest relaxation time (t) of ?-DNA (48.5 kbp) as functions of the slit height using fluorescence microscopy. Our results show that the in-plane radius of gyration increases monotonically with decreasing slit height, in contrast to results from Bonthuis et al.(15)but in agreement with our simulations and those of other groups. In strong confinement (slit height <100 nm), the scaling of D, t, and R II with slit height does not show an evident change, suggesting that the transition from the de Gennes regime to the Odijk regime is gradual and broad.

Original languageEnglish (US)
Pages (from-to)7368-7377
Number of pages10
Issue number17
StatePublished - Sep 14 2010

ASJC Scopus subject areas

  • Organic Chemistry
  • Polymers and Plastics
  • Inorganic Chemistry
  • Materials Chemistry


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