Retroviral insertions in the CB-1/Fim-3 common site of integration activate expression of the Evi-1 gene

C. Bartholomew, K. Morishita, D. Askew, A. Buchberg, N. A. Jenkins, N. G. Copeland, J. N. Ihle

Research output: Contribution to journalArticle

54 Scopus citations

Abstract

A common retroviral integration site (CB-1) was identified in two IL-3-dependent myeloid leukemia cell lines (DA-3, DA-34). The CB-1 locus was mapped to murine chromosome 3 and was shown to be closely linked to another myeloid common site of viral integrations in myeloid leukemias, Evi-1. A comparison of the CB-1 restriction map with published restriction maps for other common integration sites demonstrated that is was nearly identical to the restriction map of a common site of Friend MuLV integration in myeloid tumors termed Fim-3 (Bordereaux et al. 1987). Genomic clones representing approximately 110 kb of the CB-1 locus and 80 kb of the 5' region of the Evi-1 locus demonstrate no physical overlap of these viral integration sites. Previous studies (Morishita et al. 1988) have shown that retroviral insertions in the Evi-1 locus activate the expression of a gene potentially encoding a 120 kd zinc finger protein. Evi-1 expression is also activated in cell lines with viral integrations in the CB-1 locus. These results demonstrate that the CB-1/Fim-3 and Evi-1 loci constitute a large genomic region in which viral integrations activate the transcription of a new potential myeloid transforming gene.

Original languageEnglish (US)
Pages (from-to)529-534
Number of pages6
JournalOncogene
Volume4
Issue number5
StatePublished - 1989

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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