Retroreflective imaging system for optical labeling and detection of microorganisms

Mark H. Bergen; Jacqueline Nichols; Christopher M. Collier; Xian Jin; Balakrishnan Raja; Deborah J. Roberts; Paul Ruchhoeft; Richard C. Willson; Jonathan F. Holzman

doi:10.1364/AO.53.003647

Retroreflective imaging system for optical labeling and detection of microorganisms

Mark H. Bergen, Jacqueline Nichols, Christopher M. Collier, Xian Jin, Balakrishnan Raja, Deborah J. Roberts, Paul Ruchhoeft, Richard C. Willson, Jonathan F. Holzman

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

A retroreflective imaging system for imaging microscopic targets over macroscopic sampling areas is introduced. Detection of microorganism-bound retroreflector (RR) targets across millimeter-scale samples is implemented according to retroreflection directionality, collimation, and contrast design characteristics. Retroreflection directionality is considered for corner-cube (CC) and spherical geometries. Spherical-RRs improve directionality and reliability. Retroreflection collimation is considered for spherical-RRs. Retroreflective images for micro-CC-RRs and micro-spherical-RRs with varying refractive indices show optimal results for high refractive index BaTiO₃micro-spherical-RRs. A differential imaging technique improves retroreflection contrast by 35 dB. High refractive index micro-spherical-RRs and differential imaging, together, can detect microscopic RR targets across macroscopic areas.

Original language	English (US)
Pages (from-to)	3647-3655
Number of pages	9
Journal	Applied Optics
Issue number	17
DOIs	https://doi.org/10.1364/AO.53.003647
State	Published - Jun 10 2014

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics
Engineering (miscellaneous)
Electrical and Electronic Engineering

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10.1364/AO.53.003647

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Retroreflective imaging system for optical labeling and detection of microorganisms. / Bergen, Mark H.; Nichols, Jacqueline; Collier, Christopher M.; Jin, Xian; Raja, Balakrishnan; Roberts, Deborah J.; Ruchhoeft, Paul; Willson, Richard C.; Holzman, Jonathan F.

In: Applied Optics, No. 17, 10.06.2014, p. 3647-3655.

Research output: Contribution to journal › Article › peer-review

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abstract = "A retroreflective imaging system for imaging microscopic targets over macroscopic sampling areas is introduced. Detection of microorganism-bound retroreflector (RR) targets across millimeter-scale samples is implemented according to retroreflection directionality, collimation, and contrast design characteristics. Retroreflection directionality is considered for corner-cube (CC) and spherical geometries. Spherical-RRs improve directionality and reliability. Retroreflection collimation is considered for spherical-RRs. Retroreflective images for micro-CC-RRs and micro-spherical-RRs with varying refractive indices show optimal results for high refractive index BaTiO3micro-spherical-RRs. A differential imaging technique improves retroreflection contrast by 35 dB. High refractive index micro-spherical-RRs and differential imaging, together, can detect microscopic RR targets across macroscopic areas.",

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AU - Nichols, Jacqueline

AU - Collier, Christopher M.

AU - Jin, Xian

AU - Raja, Balakrishnan

AU - Roberts, Deborah J.

AU - Ruchhoeft, Paul

AU - Willson, Richard C.

AU - Holzman, Jonathan F.

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AB - A retroreflective imaging system for imaging microscopic targets over macroscopic sampling areas is introduced. Detection of microorganism-bound retroreflector (RR) targets across millimeter-scale samples is implemented according to retroreflection directionality, collimation, and contrast design characteristics. Retroreflection directionality is considered for corner-cube (CC) and spherical geometries. Spherical-RRs improve directionality and reliability. Retroreflection collimation is considered for spherical-RRs. Retroreflective images for micro-CC-RRs and micro-spherical-RRs with varying refractive indices show optimal results for high refractive index BaTiO3micro-spherical-RRs. A differential imaging technique improves retroreflection contrast by 35 dB. High refractive index micro-spherical-RRs and differential imaging, together, can detect microscopic RR targets across macroscopic areas.

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Retroreflective imaging system for optical labeling and detection of microorganisms

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