Abstract
We determined whether resveratrol, a phenolic anti-oxidant found in grapes and wine, inhibited phorbol ester (PMA)-mediated induction of COX-2 in human mammary and oral epithelial cells. Treatment of cells with PMA induced COX-2 mRNA and protein and caused a marked increase in PGE2 synthesis. These effects were inhibited by resveratrol. Nuclear run-offs revealed increased rates of COX-2 transcription after treatment with PMA, an effect that was inhibited by resveratrol. Resveratrol inhibited PMA-mediated activation of protein kinase C (PKC) and the induction of COX-2 promoter activity by c-Jun. Phorbol ester-mediated induction of AP-1 activity was blocked by resveratrol. In addition to the above effects on COX-2 gene expression, resveratrol also directly inhibited COX-2 enzyme activity. These data are likely to be important for understanding the anti-inflammatory and anti-cancer properties of resveratrol.
Original language | English (US) |
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Pages (from-to) | 35-43 |
Number of pages | 9 |
Journal | Pharmaceutical Biology |
Volume | 36 |
Issue number | SUPPL. |
DOIs | |
State | Published - Dec 1 1998 |
Keywords
- COX-2
- PKC
- PMA
- Resveratrol
ASJC Scopus subject areas
- Plant Science
- Medical Laboratory Technology
- Clinical Biochemistry
- Pharmacology