Resveratrol inhibits the expression of cyclooxygenase-2 in human mammary and oral epithelial cells

Kotha Subbaramaiah, Pedro Michaluart, Wen Jing Chung, Andrew Dannenberg

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

We determined whether resveratrol, a phenolic anti-oxidant found in grapes and wine, inhibited phorbol ester (PMA)-mediated induction of COX-2 in human mammary and oral epithelial cells. Treatment of cells with PMA induced COX-2 mRNA and protein and caused a marked increase in PGE2 synthesis. These effects were inhibited by resveratrol. Nuclear run-offs revealed increased rates of COX-2 transcription after treatment with PMA, an effect that was inhibited by resveratrol. Resveratrol inhibited PMA-mediated activation of protein kinase C (PKC) and the induction of COX-2 promoter activity by c-Jun. Phorbol ester-mediated induction of AP-1 activity was blocked by resveratrol. In addition to the above effects on COX-2 gene expression, resveratrol also directly inhibited COX-2 enzyme activity. These data are likely to be important for understanding the anti-inflammatory and anti-cancer properties of resveratrol.

Original languageEnglish (US)
Pages (from-to)35-43
Number of pages9
JournalPharmaceutical Biology
Volume36
Issue numberSUPPL.
DOIs
StatePublished - Dec 1 1998

Keywords

  • COX-2
  • PKC
  • PMA
  • Resveratrol

ASJC Scopus subject areas

  • Plant Science
  • Medical Laboratory Technology
  • Clinical Biochemistry
  • Pharmacology

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