Restriction map of the single-stranded DNA genome of Kilham rat virus strain 171, a nondefective parvovirus

P. T. Banerjee, R. Rothrock, S. Mitra

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

We constructed a physical map of Kilham rat virus strain 171 DNA by analyzing the sizes and locations of restriction endonuclease-generated fragments of the replicative-form viral DNA synthesized in vitro. BglI, KpnI, BamHI, SmaI, XhoI, and XorII did not appear to have any cleavage sites, whereas 11 other enzymes cleaved the genome at one to eight sites, and AluI generated more than 12 distinct fragments. The 30 restriction sites that were mapped were distributed randomly in the viral genome. A comparison of the restriction fragments of in vivo- and in vitro-replicated replicative-form DNAs showed that these DNAs were identical except in the size or configuration of the terminal fragments.

Original languageEnglish (US)
Pages (from-to)118-125
Number of pages8
JournalJournal of virology
Volume40
Issue number1
DOIs
StatePublished - 1981

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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