TY - JOUR
T1 - Resolving the graft ischemia-reperfusion injury during liver transplantation at the single cell resolution
AU - Wang, Linhe
AU - Li, Jie
AU - He, Shuai
AU - Liu, Yang
AU - Chen, Haitian
AU - He, Shujiao
AU - Yin, Meixian
AU - Zou, Dawei
AU - Chen, Shirui
AU - Luo, Tao
AU - Yu, Xinyu
AU - Wan, Xuesi
AU - Huang, Shunwei
AU - Guo, Zhiyong
AU - He, Xiaoshun
N1 - Funding Information:
This work was supported by grants as follows: the National Natural Science Foundation of China (82070670 to X.S.H. and 81970564 to Z.Y.G.), the Guangdong Provincial Key Laboratory Construction Projection on Organ Donation and Transplant Immunology (2013A061401007 and 2017B030314018 to X.S.H.), Guangdong Provincial international Cooperation Base of Science and Technology (Organ Transplantation) (2015B050501002 to X.S.H.), Science and Technology Program of Guangzhou (201704020150 to X.S.H.), Science and Technology Program of Guangdong (2020B1111140003 to X.S.H., 2016A030313239 to X.S.W.), Sun Yat-Sen University Young Teacher Key Cultivate Project (19ykzd13) and “Elite program” specially supported by China organ transplantation development foundation (2019JY02).
Publisher Copyright:
© 2021, The Author(s).
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021/6
Y1 - 2021/6
N2 - Ischemia–reperfusion injury (IRI) remains the major reason for impaired donor graft function and increased mortality post-liver transplantation. The mechanism of IRI involves multiple pathophysiological processes and numerous types of cells. However, a systematic and comprehensive single-cell transcriptional profile of intrahepatic cells during liver transplantation is still unclear. We performed a single-cell transcriptome analysis of 14,313 cells from liver tissues collected from pre-procurement, at the end of preservation and 2 h post-reperfusion. We made detailed annotations of mononuclear phagocyte, endothelial cell, NK/T, B and plasma cell clusters, and we described the dynamic changes of the transcriptome of these clusters during IRI and the interaction between mononuclear phagocyte clusters and other cell clusters. In addition, we found that TNFAIP3 interacting protein 3 (TNIP3), specifically and highly expressed in Kupffer cell clusters post-reperfusion, may have a protective effect on IRI. In summary, our study provides the first dynamic transcriptome map of intrahepatic cell clusters during liver transplantation at single-cell resolution.
AB - Ischemia–reperfusion injury (IRI) remains the major reason for impaired donor graft function and increased mortality post-liver transplantation. The mechanism of IRI involves multiple pathophysiological processes and numerous types of cells. However, a systematic and comprehensive single-cell transcriptional profile of intrahepatic cells during liver transplantation is still unclear. We performed a single-cell transcriptome analysis of 14,313 cells from liver tissues collected from pre-procurement, at the end of preservation and 2 h post-reperfusion. We made detailed annotations of mononuclear phagocyte, endothelial cell, NK/T, B and plasma cell clusters, and we described the dynamic changes of the transcriptome of these clusters during IRI and the interaction between mononuclear phagocyte clusters and other cell clusters. In addition, we found that TNFAIP3 interacting protein 3 (TNIP3), specifically and highly expressed in Kupffer cell clusters post-reperfusion, may have a protective effect on IRI. In summary, our study provides the first dynamic transcriptome map of intrahepatic cell clusters during liver transplantation at single-cell resolution.
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U2 - 10.1038/s41419-021-03878-3
DO - 10.1038/s41419-021-03878-3
M3 - Article
C2 - 34103479
AN - SCOPUS:85107566456
VL - 12
JO - Cell Death and Disease
JF - Cell Death and Disease
SN - 2041-4889
IS - 6
M1 - 589
ER -