Repression of NHE1 expression by PPARγ activation is a potential new approach for specific inhibition of the growth of tumor cells in vitro and in vivo

Alan Prem Kumar, Ai Li Quake, Michelle Ker Xing Chang, Ting Zhou, Kelly Swee Ying Lim, Rajeev Singh, Robert Edwin Hewitt, Manuel Salto-Tellez, Shazib Pervaiz, Marie Véronique Clément

Research output: Contribution to journalArticle

50 Scopus citations

Abstract

Ligand-induced activation of peroxisome proliferator-activated receptor γ (PPARγ) inhibits proliferation in cancer cells in vitro and in vivo; however, the downstream targets remain undefined. We report the identification of a peroxisome proliferator response element in the promoter region of the Na+/H+ transporter gene NHE1, the overexpression of which has been associated with carcinogenesis. Exposure of breast cancer cells expressing high levels of PPARγ to its natural and synthetic agonists resulted in downregulation of NHE1 transcription as well as protein expression. Furthermore, the inhibitory effect of activated PPARγ on tumor colony-forming ability was abrogated on overexpression of NHE1, whereas small interfering RNA-mediated gene silencing of NHE1 significantly increased the sensitivity of cancer cells to growth-inhibitory stimuli. Finally, histopathologic analysis of breast cancer biopsies obtained from patients with type II diabetes treated with the synthetic agonist rosiglitazone showed significant repression of NHE1 in the tumor tissue. These data provide evidence for tumor-selective downregulation of NHE1 by activated PPARγ in vitro and in pathologic specimens from breast cancer patients and could have potential implications for the judicious use of low doses of PPARγ ligands in combination chemotherapy regimens for an effective therapeutic response.

Original languageEnglish (US)
Pages (from-to)8636-8644
Number of pages9
JournalCancer research
Volume69
Issue number22
DOIs
StatePublished - Nov 15 2009

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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