TY - JOUR
T1 - Release of low density lipoprotein receptors from human fibroblasts or Hela cells by tryptic digestion
AU - Di Ferrante, Nicola
AU - Donnelly, Patricia V.
AU - Di Ferrante, Daniela T.
AU - Toma, Salvatore
AU - Gotto, Antonio
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1978/9/29
Y1 - 1978/9/29
N2 - Trypsin treatment of cultured normal human skin fibroblasts or Hela cells releases material which is retained on a low density lipoprotein (LDL)-Sepharose affinity column, may be eluted from it with 2.5 M KI and, after dialysis, agglutinates LDL or apo-B-coated formocells. Such agglutination is prevented by preincubation of the receptor with LDL in solution or with arginine-rich protamine. Trypsin treatment of "receptor defective" or "receptor negative" mutant fibroblasts releases material which is retained on LDL-Sepharose column but fails to agglutinate LDL-coated formocells. The receptor may be labeled with 6-[3H]-glucosamine·HCl and [3H]-leucine, it is inactivated by heating at 80°C for 10 min and may be obtained from normal fibroblasts or Hela cells, whether they were cultured in presence or in absence of lipoprotein-containing fetal calf serum.
AB - Trypsin treatment of cultured normal human skin fibroblasts or Hela cells releases material which is retained on a low density lipoprotein (LDL)-Sepharose affinity column, may be eluted from it with 2.5 M KI and, after dialysis, agglutinates LDL or apo-B-coated formocells. Such agglutination is prevented by preincubation of the receptor with LDL in solution or with arginine-rich protamine. Trypsin treatment of "receptor defective" or "receptor negative" mutant fibroblasts releases material which is retained on LDL-Sepharose column but fails to agglutinate LDL-coated formocells. The receptor may be labeled with 6-[3H]-glucosamine·HCl and [3H]-leucine, it is inactivated by heating at 80°C for 10 min and may be obtained from normal fibroblasts or Hela cells, whether they were cultured in presence or in absence of lipoprotein-containing fetal calf serum.
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U2 - 10.1016/0006-291X(78)90179-1
DO - 10.1016/0006-291X(78)90179-1
M3 - Article
C2 - 214072
AN - SCOPUS:0018074880
SN - 0006-291X
VL - 84
SP - 366
EP - 373
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -