Relationship of tissue and cellular interleukin-1 and lipopolysaccharide after endotoxemia and bacteremia

Yimin Ge; Robert M. Ezzell; Burton D. Clark; Paul M. Loiselle; Stephen F. Amato; H. Shaw Warren

doi:10.1086/514127

Relationship of tissue and cellular interleukin-1 and lipopolysaccharide after endotoxemia and bacteremia

Yimin Ge, Robert M. Ezzell, Burton D. Clark, Paul M. Loiselle, Stephen F. Amato, H. Shaw Warren

Research output: Contribution to journal › Article

36 Scopus citations

Abstract

Distribution of immunoreactive interleukin-1 (IL-1) and lipopolysaccharide (LPS) were studied in the tissues of rats after intravenous injection of purified LPS or live Escherichia coli bacteria. IL- 1 staining in the spleen peaked at 4-8 h, colocalized with LPS in marginal zone macrophages, and was undetectable 24 h after injection, whereas LPS staining peaked at 24 h and was detectable for 4 weeks. The tissue IL-1 response was similar for LPS and live bacteria. Thus, tissue IL-1 is down- regulated within hours despite maintenance of LPS in the same cells for weeks. Macrophages in liver and lung had only slight IL-1 staining despite intense staining for LPS. Tissue IL-1 production appears to be differentially regulated after gram-negative bacteremia; LPS cleared by liver and lung macrophages elicit minimal IL-1, whereas there is high local IL-1 production in the marginal zone of the spleen that may increase immune responses to bacterial wall antigens.

Original language	English (US)
Pages (from-to)	1313-1321
Number of pages	9
Journal	Journal of Infectious Diseases
Volume	176
Issue number	5
DOIs	https://doi.org/10.1086/514127
State	Published - 1997

ASJC Scopus subject areas

Immunology and Allergy
Infectious Diseases

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10.1086/514127

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title = "Relationship of tissue and cellular interleukin-1 and lipopolysaccharide after endotoxemia and bacteremia",

abstract = "Distribution of immunoreactive interleukin-1 (IL-1) and lipopolysaccharide (LPS) were studied in the tissues of rats after intravenous injection of purified LPS or live Escherichia coli bacteria. IL- 1 staining in the spleen peaked at 4-8 h, colocalized with LPS in marginal zone macrophages, and was undetectable 24 h after injection, whereas LPS staining peaked at 24 h and was detectable for 4 weeks. The tissue IL-1 response was similar for LPS and live bacteria. Thus, tissue IL-1 is down- regulated within hours despite maintenance of LPS in the same cells for weeks. Macrophages in liver and lung had only slight IL-1 staining despite intense staining for LPS. Tissue IL-1 production appears to be differentially regulated after gram-negative bacteremia; LPS cleared by liver and lung macrophages elicit minimal IL-1, whereas there is high local IL-1 production in the marginal zone of the spleen that may increase immune responses to bacterial wall antigens.",

author = "Yimin Ge and Ezzell, {Robert M.} and Clark, {Burton D.} and Loiselle, {Paul M.} and Amato, {Stephen F.} and {Shaw Warren}, H.",

year = "1997",

doi = "10.1086/514127",

language = "English (US)",

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pages = "1313--1321",

journal = "Journal of Infectious Diseases",

issn = "0022-1899",

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T1 - Relationship of tissue and cellular interleukin-1 and lipopolysaccharide after endotoxemia and bacteremia

AU - Ge, Yimin

AU - Ezzell, Robert M.

AU - Clark, Burton D.

AU - Loiselle, Paul M.

AU - Amato, Stephen F.

AU - Shaw Warren, H.

PY - 1997

Y1 - 1997

N2 - Distribution of immunoreactive interleukin-1 (IL-1) and lipopolysaccharide (LPS) were studied in the tissues of rats after intravenous injection of purified LPS or live Escherichia coli bacteria. IL- 1 staining in the spleen peaked at 4-8 h, colocalized with LPS in marginal zone macrophages, and was undetectable 24 h after injection, whereas LPS staining peaked at 24 h and was detectable for 4 weeks. The tissue IL-1 response was similar for LPS and live bacteria. Thus, tissue IL-1 is down- regulated within hours despite maintenance of LPS in the same cells for weeks. Macrophages in liver and lung had only slight IL-1 staining despite intense staining for LPS. Tissue IL-1 production appears to be differentially regulated after gram-negative bacteremia; LPS cleared by liver and lung macrophages elicit minimal IL-1, whereas there is high local IL-1 production in the marginal zone of the spleen that may increase immune responses to bacterial wall antigens.

AB - Distribution of immunoreactive interleukin-1 (IL-1) and lipopolysaccharide (LPS) were studied in the tissues of rats after intravenous injection of purified LPS or live Escherichia coli bacteria. IL- 1 staining in the spleen peaked at 4-8 h, colocalized with LPS in marginal zone macrophages, and was undetectable 24 h after injection, whereas LPS staining peaked at 24 h and was detectable for 4 weeks. The tissue IL-1 response was similar for LPS and live bacteria. Thus, tissue IL-1 is down- regulated within hours despite maintenance of LPS in the same cells for weeks. Macrophages in liver and lung had only slight IL-1 staining despite intense staining for LPS. Tissue IL-1 production appears to be differentially regulated after gram-negative bacteremia; LPS cleared by liver and lung macrophages elicit minimal IL-1, whereas there is high local IL-1 production in the marginal zone of the spleen that may increase immune responses to bacterial wall antigens.

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