Relationship of tissue and cellular interleukin-1 and lipopolysaccharide after endotoxemia and bacteremia

Yimin Ge; Robert M. Ezzell; Burton D. Clark; Paul M. Loiselle; Stephen F. Amato; H. Shaw Warren

doi:10.1086/514127

Relationship of tissue and cellular interleukin-1 and lipopolysaccharide after endotoxemia and bacteremia

Yimin Ge, Robert M. Ezzell, Burton D. Clark, Paul M. Loiselle, Stephen F. Amato, H. Shaw Warren

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

Distribution of immunoreactive interleukin-1 (IL-1) and lipopolysaccharide (LPS) were studied in the tissues of rats after intravenous injection of purified LPS or live Escherichia coli bacteria. IL- 1 staining in the spleen peaked at 4-8 h, colocalized with LPS in marginal zone macrophages, and was undetectable 24 h after injection, whereas LPS staining peaked at 24 h and was detectable for 4 weeks. The tissue IL-1 response was similar for LPS and live bacteria. Thus, tissue IL-1 is down- regulated within hours despite maintenance of LPS in the same cells for weeks. Macrophages in liver and lung had only slight IL-1 staining despite intense staining for LPS. Tissue IL-1 production appears to be differentially regulated after gram-negative bacteremia; LPS cleared by liver and lung macrophages elicit minimal IL-1, whereas there is high local IL-1 production in the marginal zone of the spleen that may increase immune responses to bacterial wall antigens.

Original language	English (US)
Pages (from-to)	1313-1321
Number of pages	9
Journal	Journal of Infectious Diseases
Volume	176
Issue number	5
DOIs	https://doi.org/10.1086/514127
State	Published - 1997

ASJC Scopus subject areas

Immunology and Allergy
Infectious Diseases

Access to Document

10.1086/514127

Cite this

@article{659ad1a9693448b19c0dcd3b8758c293,

title = "Relationship of tissue and cellular interleukin-1 and lipopolysaccharide after endotoxemia and bacteremia",

abstract = "Distribution of immunoreactive interleukin-1 (IL-1) and lipopolysaccharide (LPS) were studied in the tissues of rats after intravenous injection of purified LPS or live Escherichia coli bacteria. IL- 1 staining in the spleen peaked at 4-8 h, colocalized with LPS in marginal zone macrophages, and was undetectable 24 h after injection, whereas LPS staining peaked at 24 h and was detectable for 4 weeks. The tissue IL-1 response was similar for LPS and live bacteria. Thus, tissue IL-1 is down- regulated within hours despite maintenance of LPS in the same cells for weeks. Macrophages in liver and lung had only slight IL-1 staining despite intense staining for LPS. Tissue IL-1 production appears to be differentially regulated after gram-negative bacteremia; LPS cleared by liver and lung macrophages elicit minimal IL-1, whereas there is high local IL-1 production in the marginal zone of the spleen that may increase immune responses to bacterial wall antigens.",

author = "Yimin Ge and Ezzell, {Robert M.} and Clark, {Burton D.} and Loiselle, {Paul M.} and Amato, {Stephen F.} and {Shaw Warren}, H.",

note = "Funding Information: Received 30 December 1996; revised 19 June 1997. Grant support: Shriners Hospital for Crippled Children (15810), Department of the Navy, Office of the Chief of Naval Research (N0014-94-C-0021), and NIH (AI-28943, GM-21700, GM-50766). Reprints or correspondence: Dr. H. Shaw Warren, Infectious Disease Unit, 5th Floor, Massachusetts General Hospital, 149 13th St., Charlestown, MA 02129. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.",

year = "1997",

doi = "10.1086/514127",

language = "English (US)",

volume = "176",

pages = "1313--1321",

journal = "Journal of Infectious Diseases",

issn = "0022-1899",

publisher = "Oxford University Press",

number = "5",

}

TY - JOUR

T1 - Relationship of tissue and cellular interleukin-1 and lipopolysaccharide after endotoxemia and bacteremia

AU - Ge, Yimin

AU - Ezzell, Robert M.

AU - Clark, Burton D.

AU - Loiselle, Paul M.

AU - Amato, Stephen F.

AU - Shaw Warren, H.

N1 - Funding Information: Received 30 December 1996; revised 19 June 1997. Grant support: Shriners Hospital for Crippled Children (15810), Department of the Navy, Office of the Chief of Naval Research (N0014-94-C-0021), and NIH (AI-28943, GM-21700, GM-50766). Reprints or correspondence: Dr. H. Shaw Warren, Infectious Disease Unit, 5th Floor, Massachusetts General Hospital, 149 13th St., Charlestown, MA 02129. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.

PY - 1997

Y1 - 1997

N2 - Distribution of immunoreactive interleukin-1 (IL-1) and lipopolysaccharide (LPS) were studied in the tissues of rats after intravenous injection of purified LPS or live Escherichia coli bacteria. IL- 1 staining in the spleen peaked at 4-8 h, colocalized with LPS in marginal zone macrophages, and was undetectable 24 h after injection, whereas LPS staining peaked at 24 h and was detectable for 4 weeks. The tissue IL-1 response was similar for LPS and live bacteria. Thus, tissue IL-1 is down- regulated within hours despite maintenance of LPS in the same cells for weeks. Macrophages in liver and lung had only slight IL-1 staining despite intense staining for LPS. Tissue IL-1 production appears to be differentially regulated after gram-negative bacteremia; LPS cleared by liver and lung macrophages elicit minimal IL-1, whereas there is high local IL-1 production in the marginal zone of the spleen that may increase immune responses to bacterial wall antigens.

AB - Distribution of immunoreactive interleukin-1 (IL-1) and lipopolysaccharide (LPS) were studied in the tissues of rats after intravenous injection of purified LPS or live Escherichia coli bacteria. IL- 1 staining in the spleen peaked at 4-8 h, colocalized with LPS in marginal zone macrophages, and was undetectable 24 h after injection, whereas LPS staining peaked at 24 h and was detectable for 4 weeks. The tissue IL-1 response was similar for LPS and live bacteria. Thus, tissue IL-1 is down- regulated within hours despite maintenance of LPS in the same cells for weeks. Macrophages in liver and lung had only slight IL-1 staining despite intense staining for LPS. Tissue IL-1 production appears to be differentially regulated after gram-negative bacteremia; LPS cleared by liver and lung macrophages elicit minimal IL-1, whereas there is high local IL-1 production in the marginal zone of the spleen that may increase immune responses to bacterial wall antigens.

UR - http://www.scopus.com/inward/record.url?scp=0030830226&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030830226&partnerID=8YFLogxK

U2 - 10.1086/514127

DO - 10.1086/514127

M3 - Article

C2 - 9359733

AN - SCOPUS:0030830226

VL - 176

SP - 1313

EP - 1321

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

SN - 0022-1899

IS - 5

ER -

Relationship of tissue and cellular interleukin-1 and lipopolysaccharide after endotoxemia and bacteremia

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this