Relation between digitalis binding in vivo and inhibition of sodium, potassium-adenosine triphosphatase in canine kidney

Julius C. Allen, M. Martinez-Maldonado, G. Eknoyan, Wadi N. Suki, Arnold Schwartz

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

Digoxin was infused into a dog renal artery, using the contralateral kidney as control. At peak diuretic effect, sodium, potassium-adenosine triphosphatase (Na+,K+-ATPase) activity of both experimental cortex and medulla was significantly depressed compared to control. The inhibitory effect in vitro of ouabain on the control Na+,K+-ATPase from both medulla and cortex was time dependent; no regional difference in sensitivity was noted. The binding in vitro of 3H-ouabain to Na+, K+-ATPase from both experimental cortex and experimental medulla was significantly lower than that for control enzymes, suggesting previous occupation of drug-binding sites. The data represent further indication that digoxin-induced diuresis is related to inhibition of Na+,K+-ATPase.

Original languageEnglish (US)
Pages (from-to)73-80
Number of pages8
JournalBiochemical pharmacology
Volume20
Issue number1
DOIs
StatePublished - Jan 1971

ASJC Scopus subject areas

  • Biochemistry
  • Pharmacology

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