Reimaging biological barriers affecting distribution and extravasation of PEG/peptide- modified liposomes in xenograft SMMC7721 tumor

Hailing Tang, Mengjie Rui, Junhua Mai, Wei Guo, Yuhong Xu

Research output: Contribution to journalArticle

Abstract

Liposomes, as one of the most successful nanotherapeutics, have a major impact on many biomedical areas. In this study, we performed laser scanning confocal microscope (LSCM) and immunohistochemistry (IHC) assays to investigate the intra-tumor transport and antitumor mechanism of GE11 peptide-conjugated active targeting liposomes (GE11-TLs) in SMMC7721 xenograft model. According to classification of individual cell types in high resolution images, biodistribution of macrophages, tumor cells, cells with high epidermal growth factor receptor (EGFR) expression and interstitial matrix in tumor microenvironment, in addition, their impacts on intra-tumor penetration of GE11-TLs were estimated. Type I collagen fibers and macrophage flooded in the whole SMMC7721 tumor xenografts. Tumor angiogenesis was of great heterogeneity from the periphery to the center region. However, the receptor-binding site barriers were supposed to be the leading cause of poor penetration of GE11-TLs. We anticipate these images can give a deep reconsideration for rational design of target nanoparticles for overcoming biological barriers to drug delivery.

Original languageEnglish (US)
Pages (from-to)546-556
Number of pages11
JournalActa Pharmaceutica Sinica B
Volume10
Issue number3
DOIs
StatePublished - Mar 2020

Keywords

  • Biology barrier
  • EGFR
  • GE11
  • Liposome
  • SMMC7721
  • Target delivery

ASJC Scopus subject areas

  • Pharmacology, Toxicology and Pharmaceutics(all)

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