Regulation of the lifespan in dendritic cell subsets

Min Chen, Li Huang, Zainuer Shabier, Jin Wang

Research output: Contribution to journalArticle

63 Scopus citations

Abstract

The lifespan of dendritic cells (DCs) can potentially influence immune responses by affecting the duration of DCs in stimulating lymphocytes. Significant differences in the lifespan have been reported for various DC subsets, however, the molecular mechanisms for regulating such differences between DC subsets remain unclear. In this study, we compared the apoptosis signaling molecules in two major DC subjects, the myeloidSave DCs (mDCs) and plasmacytoid DCs (pDCs). We observed a lower ratio between anti-apoptotic Bcl-2/Bcl-xL and pro-apoptotic Bax/Bak in shorter-lived myeloid DCs (mDCs) than in longer-lived plasmacytoid DCs (pDCs) or T cells. Transfection with Bcl-2 or Bcl-xL prolonged the survival of mouse primary mDCs in vitro, while deletion of Bcl-2 accelerated DC turnover in vivo. In addition, the ratios between anti-apoptotic Bcl-2/Bcl-xL and pro-apoptotic Bax/Bak could be regulated in DCs. Signaling from toll-like receptors (TLRs) up-regulated Bcl-xL and improved DC survival. Our data suggest that differential expression of apoptosis signaling molecules regulates the lifespan of different DC subsets.

Original languageEnglish (US)
Pages (from-to)2558-65
Number of pages8
JournalMolecular Immunology
Volume44
Issue number10
DOIs
StatePublished - Apr 2007

Keywords

  • Animals
  • Apoptosis
  • Apoptosis Regulatory Proteins
  • Cell Survival
  • Dendritic Cells
  • Female
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-bcl-2
  • Signal Transduction
  • T-Lymphocytes
  • bcl-2 Homologous Antagonist-Killer Protein
  • bcl-2-Associated X Protein
  • bcl-X Protein
  • Journal Article
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

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