Regulation of subnuclear localization is associated with a mechanism for nuclear receptor corepression by RIP140

Hiroshi Tazawa, Waffa Osman, Yutaka Shoji, Eckardt Treuter, Jan Åke Gustafsson, Johanna Zilliacus

Research output: Contribution to journalArticle

47 Scopus citations

Abstract

Regulation of gene transcription by nuclear receptors involves association with numerous coregulators. Receptor-interacting protein 140 (RIP140) is a corepressor that negatively regulates the ligand-induced activity of several nuclear receptors, including the glucocorticoid receptor (GR). In the present study, we have characterized the role of the intranuclear localization of RIP140 in its corepressor activity. In the absence of ligand-activated GR, RIP140 is localized in small nuclear foci targeted by a 40-amino-acid-long sequence. Although the focus-targeting domain overlaps with a binding sequence for the corepressor CtBP (C-terminal binding protein), interaction with CtBP is not involved in the localization. RIP140 foci do not correspond to PML bodies but partly colocalize with domains harboring the corepressor SMRT. Upon ligand binding, GR and RIP140 are redistributed to large nuclear domains distinct from the RIP140 foci. The redistribution requires regions of RIP140 with corepressor activity, as well as the DNA-binding domain of GR. Furthermore, we show that full RIP140 corepressor activity is contributed both by C-terminal receptor-binding LXXLL motifs and interaction with the CtBP corepressor. In conclusion, our results suggest that the corepressor function of RIP140 is multifaceted and involves binding to nuclear receptors, as well as additional functions mediated by the formation and intranuclear relocalization of a repressive protein complex.

Original languageEnglish (US)
Pages (from-to)4187-4198
Number of pages12
JournalMolecular and Cellular Biology
Volume23
Issue number12
DOIs
StatePublished - Jun 2003

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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