Redifferentiation of human hepatoma cells (SMMC-7721) induced by two new highly oxygenated bisabolane-type sesquiterpenes

Ruidong Miao; Juan Wei; Qi Zhang; Venkateswara Sajja; Jinbo Yang; Qin Wang

doi:10.1007/s12038-008-0092-x

Redifferentiation of human hepatoma cells (SMMC-7721) induced by two new highly oxygenated bisabolane-type sesquiterpenes

Ruidong Miao, Juan Wei, Qi Zhang, Venkateswara Sajja, Jinbo Yang, Qin Wang

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Bisabolane-type sesquiterpenes are a class of biologically active compounds that has antitumour, antifungal, antibacterial, antioxidant and antivenom properties. We investigated the effect of two new highly oxygenated bisabolane-type sesquiterpenes (HOBS) isolated from Cremanthodium discoideum (C. discoideum) on tumour cells. Our results showed that HOBS induced morphological differentiation and reduced microvilli formation on the cell surface in SMMC-7721 cells. Flow cytometry analysis demonstrated that HOBS could induce cell-cycle arrest in the G1 phase. Moreover, HOBS was able to increase tyrosine-α-ketoglutarate transaminase activity, decrease α foetoprotein level and γ-glutamyl transferase activity. In addition, we found that HOBS inhibited the anchorageindependent growth of SMMC-7721 cells in a dose-dependent manner. Taken together, all the above observations indicate that HOBS might be able to normalize malignant SMMC-7721 cells by inhibiting cell proliferation and inducing redifferentiation.

Original language	English (US)
Pages (from-to)	723-730
Number of pages	8
Journal	Journal of Biosciences
Volume	33
Issue number	5
DOIs	https://doi.org/10.1007/s12038-008-0092-x
State	Published - Dec 2008

Keywords

Oxygenated bisabolane-type sesquiterpenes (HOBS)
Proliferation
Redifferentiation
Tumour

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Agricultural and Biological Sciences(all)

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10.1007/s12038-008-0092-x

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title = "Redifferentiation of human hepatoma cells (SMMC-7721) induced by two new highly oxygenated bisabolane-type sesquiterpenes",

abstract = "Bisabolane-type sesquiterpenes are a class of biologically active compounds that has antitumour, antifungal, antibacterial, antioxidant and antivenom properties. We investigated the effect of two new highly oxygenated bisabolane-type sesquiterpenes (HOBS) isolated from Cremanthodium discoideum (C. discoideum) on tumour cells. Our results showed that HOBS induced morphological differentiation and reduced microvilli formation on the cell surface in SMMC-7721 cells. Flow cytometry analysis demonstrated that HOBS could induce cell-cycle arrest in the G1 phase. Moreover, HOBS was able to increase tyrosine-α-ketoglutarate transaminase activity, decrease α foetoprotein level and γ-glutamyl transferase activity. In addition, we found that HOBS inhibited the anchorageindependent growth of SMMC-7721 cells in a dose-dependent manner. Taken together, all the above observations indicate that HOBS might be able to normalize malignant SMMC-7721 cells by inhibiting cell proliferation and inducing redifferentiation.",

keywords = "Oxygenated bisabolane-type sesquiterpenes (HOBS), Proliferation, Redifferentiation, Tumour",

author = "Ruidong Miao and Juan Wei and Qi Zhang and Venkateswara Sajja and Jinbo Yang and Qin Wang",

note = "Funding Information: We thank Drs Jia Zhongjian and Zhu Ying for providing HOBS. We also thank Dr Nathan Sears for critical reading and helpful discussion. This work was supported by a grant from The International Cooperation Item of Gansu Province (No. 4WSO54-A75-036). Copyright: Copyright 2009 Elsevier B.V., All rights reserved.",

year = "2008",

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doi = "10.1007/s12038-008-0092-x",

language = "English (US)",

volume = "33",

pages = "723--730",

journal = "Journal of Biosciences",

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AU - Miao, Ruidong

AU - Wei, Juan

AU - Zhang, Qi

AU - Sajja, Venkateswara

AU - Yang, Jinbo

AU - Wang, Qin

N1 - Funding Information: We thank Drs Jia Zhongjian and Zhu Ying for providing HOBS. We also thank Dr Nathan Sears for critical reading and helpful discussion. This work was supported by a grant from The International Cooperation Item of Gansu Province (No. 4WSO54-A75-036). Copyright: Copyright 2009 Elsevier B.V., All rights reserved.

PY - 2008/12

Y1 - 2008/12

N2 - Bisabolane-type sesquiterpenes are a class of biologically active compounds that has antitumour, antifungal, antibacterial, antioxidant and antivenom properties. We investigated the effect of two new highly oxygenated bisabolane-type sesquiterpenes (HOBS) isolated from Cremanthodium discoideum (C. discoideum) on tumour cells. Our results showed that HOBS induced morphological differentiation and reduced microvilli formation on the cell surface in SMMC-7721 cells. Flow cytometry analysis demonstrated that HOBS could induce cell-cycle arrest in the G1 phase. Moreover, HOBS was able to increase tyrosine-α-ketoglutarate transaminase activity, decrease α foetoprotein level and γ-glutamyl transferase activity. In addition, we found that HOBS inhibited the anchorageindependent growth of SMMC-7721 cells in a dose-dependent manner. Taken together, all the above observations indicate that HOBS might be able to normalize malignant SMMC-7721 cells by inhibiting cell proliferation and inducing redifferentiation.

AB - Bisabolane-type sesquiterpenes are a class of biologically active compounds that has antitumour, antifungal, antibacterial, antioxidant and antivenom properties. We investigated the effect of two new highly oxygenated bisabolane-type sesquiterpenes (HOBS) isolated from Cremanthodium discoideum (C. discoideum) on tumour cells. Our results showed that HOBS induced morphological differentiation and reduced microvilli formation on the cell surface in SMMC-7721 cells. Flow cytometry analysis demonstrated that HOBS could induce cell-cycle arrest in the G1 phase. Moreover, HOBS was able to increase tyrosine-α-ketoglutarate transaminase activity, decrease α foetoprotein level and γ-glutamyl transferase activity. In addition, we found that HOBS inhibited the anchorageindependent growth of SMMC-7721 cells in a dose-dependent manner. Taken together, all the above observations indicate that HOBS might be able to normalize malignant SMMC-7721 cells by inhibiting cell proliferation and inducing redifferentiation.

KW - Oxygenated bisabolane-type sesquiterpenes (HOBS)

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KW - Redifferentiation

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Redifferentiation of human hepatoma cells (SMMC-7721) induced by two new highly oxygenated bisabolane-type sesquiterpenes

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