TY - JOUR
T1 - Redifferentiation of human hepatoma cells (SMMC-7721) induced by two new highly oxygenated bisabolane-type sesquiterpenes
AU - Miao, Ruidong
AU - Wei, Juan
AU - Zhang, Qi
AU - Sajja, Venkateswara
AU - Yang, Jinbo
AU - Wang, Qin
N1 - Funding Information:
We thank Drs Jia Zhongjian and Zhu Ying for providing HOBS. We also thank Dr Nathan Sears for critical reading and helpful discussion. This work was supported by a grant from The International Cooperation Item of Gansu Province (No. 4WSO54-A75-036).
Copyright:
Copyright 2009 Elsevier B.V., All rights reserved.
PY - 2008/12
Y1 - 2008/12
N2 - Bisabolane-type sesquiterpenes are a class of biologically active compounds that has antitumour, antifungal, antibacterial, antioxidant and antivenom properties. We investigated the effect of two new highly oxygenated bisabolane-type sesquiterpenes (HOBS) isolated from Cremanthodium discoideum (C. discoideum) on tumour cells. Our results showed that HOBS induced morphological differentiation and reduced microvilli formation on the cell surface in SMMC-7721 cells. Flow cytometry analysis demonstrated that HOBS could induce cell-cycle arrest in the G1 phase. Moreover, HOBS was able to increase tyrosine-α-ketoglutarate transaminase activity, decrease α foetoprotein level and γ-glutamyl transferase activity. In addition, we found that HOBS inhibited the anchorageindependent growth of SMMC-7721 cells in a dose-dependent manner. Taken together, all the above observations indicate that HOBS might be able to normalize malignant SMMC-7721 cells by inhibiting cell proliferation and inducing redifferentiation.
AB - Bisabolane-type sesquiterpenes are a class of biologically active compounds that has antitumour, antifungal, antibacterial, antioxidant and antivenom properties. We investigated the effect of two new highly oxygenated bisabolane-type sesquiterpenes (HOBS) isolated from Cremanthodium discoideum (C. discoideum) on tumour cells. Our results showed that HOBS induced morphological differentiation and reduced microvilli formation on the cell surface in SMMC-7721 cells. Flow cytometry analysis demonstrated that HOBS could induce cell-cycle arrest in the G1 phase. Moreover, HOBS was able to increase tyrosine-α-ketoglutarate transaminase activity, decrease α foetoprotein level and γ-glutamyl transferase activity. In addition, we found that HOBS inhibited the anchorageindependent growth of SMMC-7721 cells in a dose-dependent manner. Taken together, all the above observations indicate that HOBS might be able to normalize malignant SMMC-7721 cells by inhibiting cell proliferation and inducing redifferentiation.
KW - Oxygenated bisabolane-type sesquiterpenes (HOBS)
KW - Proliferation
KW - Redifferentiation
KW - Tumour
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U2 - 10.1007/s12038-008-0092-x
DO - 10.1007/s12038-008-0092-x
M3 - Article
C2 - 19179760
AN - SCOPUS:59449084540
SN - 0250-5991
VL - 33
SP - 723
EP - 730
JO - Journal of Biosciences
JF - Journal of Biosciences
IS - 5
ER -