TY - JOUR
T1 - Redifferentiation of human hepatoma cells induced by synthesized coumarin
AU - Pan, Jing
AU - Zhang, Qi
AU - Zhao, Chen Yang
AU - Zheng, Rong Liang
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2004/5
Y1 - 2004/5
N2 - The effects of synthesized 7-OH-4-CH3-coumarin on proliferation and differentiation of human hepatoma carcinoma cell line, SMMC-7721, were examined. Results showed that 7-OH-4-CH3-coumarin suppressed the proliferation of the SMMC-7721 cells in a dose-dependent manner, with an IC 50 value of 356±1.8mM, while concentrations≤200mM could trigger differentiation. After treatment with this compound at 100mM, the growth curve of human hepatoma cells decreased markedly. When treated with 50 and 100mM, the cells' electrophoresis rate decreased from 2.2mm/s/V/cm in the control group to 1.5 and 1.8mm/s/V/cm, respectively, and the α-fetoprotein content decreased from 123ng/mg in the control group to 68 and 45ng/mg, respectively. The microvilli on the surface of treated cells were also reduced. All the above indexes related to cell malignancy were alleviated significantly. Results showed that 7-OH-4-CH3-coumarin could reverse human hepatoma cells' malignant phenotypic characteristics and induce redifferentiation.
AB - The effects of synthesized 7-OH-4-CH3-coumarin on proliferation and differentiation of human hepatoma carcinoma cell line, SMMC-7721, were examined. Results showed that 7-OH-4-CH3-coumarin suppressed the proliferation of the SMMC-7721 cells in a dose-dependent manner, with an IC 50 value of 356±1.8mM, while concentrations≤200mM could trigger differentiation. After treatment with this compound at 100mM, the growth curve of human hepatoma cells decreased markedly. When treated with 50 and 100mM, the cells' electrophoresis rate decreased from 2.2mm/s/V/cm in the control group to 1.5 and 1.8mm/s/V/cm, respectively, and the α-fetoprotein content decreased from 123ng/mg in the control group to 68 and 45ng/mg, respectively. The microvilli on the surface of treated cells were also reduced. All the above indexes related to cell malignancy were alleviated significantly. Results showed that 7-OH-4-CH3-coumarin could reverse human hepatoma cells' malignant phenotypic characteristics and induce redifferentiation.
KW - Coumarin
KW - Hepatoma cell
KW - Proliferation
KW - Reactive oxygen species
KW - Redifferentiation
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UR - http://www.scopus.com/inward/citedby.url?scp=2942582872&partnerID=8YFLogxK
U2 - 10.1016/j.cellbi.2004.02.002
DO - 10.1016/j.cellbi.2004.02.002
M3 - Article
C2 - 15193276
AN - SCOPUS:2942582872
SN - 1065-6995
VL - 28
SP - 329
EP - 333
JO - Cell Biology International
JF - Cell Biology International
IS - 5
ER -