Recruitment of the SWI-SNF chromatin remodeling complex as a mechanism of gene activation by the glucocorticoid receptor τ1 activation domain

Annika E. Wallberg, Kristen E. Neely, Ahmed H. Hassan, Jan åke Gustafsson, Jerry L. Workman, Anthony P.H. Wright

Research output: Contribution to journalArticle

104 Scopus citations

Abstract

The SWI-SNF complex has been shown to alter nucleosome conformation in an ATP-dependent manner, leading to increased accessibility of nucleosomal DNA to transcription factors. In this study, we show that the SWI-SNF complex can potentiate the activity of the glucocorticoid receptor (GR) through the N-terminal transactivation domain, τ1, in both yeast and mammalian cells. GR-τ1 can directly interact with purified SWI-SNF complex, and mutations in τ1 that affect the transactivation activity in vivo also directly affect τ1 interaction with SWI-SNF. Furthermore, the SWI-SNF complex can stimulate τ1- driven transcription from chromatin templates in vitro. Taken together, these results support a model in which the GR can directly recruit the SWI-SNF complex to target promoters during glucocorticoid-dependent gene activation. We also provide evidence that the SWI-SNF and SAGA complexes represent independent pathways of τ1-mediated activation but play overlapping roles that are able to compensate for one another under some conditions.

Original languageEnglish (US)
Pages (from-to)2004-2013
Number of pages10
JournalMolecular and Cellular Biology
Volume20
Issue number6
DOIs
StatePublished - Mar 2000

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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