Recombineering: A powerful new tool for mouse functional genomics

Research output: Contribution to journalReview article

565 Scopus citations

Abstract

Highly efficient phage-based Escherichia coli homologous recombination systems have recently been developed that enable genomic DNA in bacterial artificial chromosomes to be modified and subcloned, without the need for restriction enzymes or DNA ligases. This new form of chromosome engineering, termed recombinogenic engineering or recombineering, is efficient and greatly decreases the time it takes to create transgenic mouse models by traditional means. Recombineering also facilitates many kinds of genomic experiment that have otherwise been difficult to carry out, and should enhance functional genomic studies by providing better mouse models and a more refined genetic analysis of the mouse genome.

Original languageEnglish (US)
Pages (from-to)769-779
Number of pages11
JournalNature Reviews Genetics
Volume2
Issue number10
DOIs
StatePublished - Oct 1 2001

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

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