Receptor-mediated delivery of siRNAs by tethered nucleic acid base-paired interactions

Kexiong Zhang; Qiaoqiao Wang; Yanhua Xie; Gil Mor; Emanuela Sega; Philip S. Low; Yingqun Huang

doi:10.1261/rna.739308

Receptor-mediated delivery of siRNAs by tethered nucleic acid base-paired interactions

Kexiong Zhang, Qiaoqiao Wang, Yanhua Xie, Gil Mor, Emanuela Sega, Philip S. Low, Yingqun Huang

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

We report a new strategy for cell-type-specific delivery of functional siRNAs into cells. The method involves the noncovalent attachment of siRNAs to ligand-conjugated oligodeoxynucleotides via nucleic acid base-paired interactions. The resulting complexes can be directly applied to cells, leading to specific cellular uptake and gene silencing. The method is simple, economical, and can be easily adapted for other cell surface receptors. Here we show the application of this method for the delivery of siRNAs to folate receptor-expressing cells. Published by Cold Spring Harbor Laboratory Press.

Original language	English (US)
Pages (from-to)	577-583
Number of pages	7
Journal	RNA
Volume	14
Issue number	3
DOIs	https://doi.org/10.1261/rna.739308
State	Published - Mar 2008

Keywords

Folate receptor
Gene therapy
siRNA delivery

ASJC Scopus subject areas

Genetics
Molecular Biology
General Medicine

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10.1261/rna.739308

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abstract = "We report a new strategy for cell-type-specific delivery of functional siRNAs into cells. The method involves the noncovalent attachment of siRNAs to ligand-conjugated oligodeoxynucleotides via nucleic acid base-paired interactions. The resulting complexes can be directly applied to cells, leading to specific cellular uptake and gene silencing. The method is simple, economical, and can be easily adapted for other cell surface receptors. Here we show the application of this method for the delivery of siRNAs to folate receptor-expressing cells. Published by Cold Spring Harbor Laboratory Press.",

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AU - Zhang, Kexiong

AU - Wang, Qiaoqiao

AU - Xie, Yanhua

AU - Mor, Gil

AU - Sega, Emanuela

AU - Low, Philip S.

AU - Huang, Yingqun

PY - 2008/3

Y1 - 2008/3

N2 - We report a new strategy for cell-type-specific delivery of functional siRNAs into cells. The method involves the noncovalent attachment of siRNAs to ligand-conjugated oligodeoxynucleotides via nucleic acid base-paired interactions. The resulting complexes can be directly applied to cells, leading to specific cellular uptake and gene silencing. The method is simple, economical, and can be easily adapted for other cell surface receptors. Here we show the application of this method for the delivery of siRNAs to folate receptor-expressing cells. Published by Cold Spring Harbor Laboratory Press.

AB - We report a new strategy for cell-type-specific delivery of functional siRNAs into cells. The method involves the noncovalent attachment of siRNAs to ligand-conjugated oligodeoxynucleotides via nucleic acid base-paired interactions. The resulting complexes can be directly applied to cells, leading to specific cellular uptake and gene silencing. The method is simple, economical, and can be easily adapted for other cell surface receptors. Here we show the application of this method for the delivery of siRNAs to folate receptor-expressing cells. Published by Cold Spring Harbor Laboratory Press.

KW - Folate receptor

KW - Gene therapy

KW - siRNA delivery

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Receptor-mediated delivery of siRNAs by tethered nucleic acid base-paired interactions

Abstract

Keywords

ASJC Scopus subject areas

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