Receptor-mediated delivery of siRNAs by tethered nucleic acid base-paired interactions

Kexiong Zhang, Qiaoqiao Wang, Yanhua Xie, Gil Mor, Emanuela Sega, Philip S. Low, Yingqun Huang

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

We report a new strategy for cell-type-specific delivery of functional siRNAs into cells. The method involves the noncovalent attachment of siRNAs to ligand-conjugated oligodeoxynucleotides via nucleic acid base-paired interactions. The resulting complexes can be directly applied to cells, leading to specific cellular uptake and gene silencing. The method is simple, economical, and can be easily adapted for other cell surface receptors. Here we show the application of this method for the delivery of siRNAs to folate receptor-expressing cells. Published by Cold Spring Harbor Laboratory Press.

Original languageEnglish (US)
Pages (from-to)577-583
Number of pages7
JournalRNA
Volume14
Issue number3
DOIs
StatePublished - Mar 2008

Keywords

  • Folate receptor
  • Gene therapy
  • siRNA delivery

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Medicine(all)

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