Reaction of human macrophages on protein corona covered TiO2 nanoparticles

Camila F. Borgognoni, Michael Mormann, Ying Qu, Marcus Schäfer, Klaus Langer, Cengiz Öztürk, Sylvia Wagner, Chunying Chen, Yuliang Zhao, Harald Fuchs, Kristina Riehemann

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

The cytokine secretion of primary cells of human macrophages during the interaction of TiO2 nanoparticles (with an average primary size of 100-120nm) is investigated down to concentration levels suggested to be relevant for in vivo conditions. We find that high TiO2 concentrations induce the cytokines Interleukin IL-1β, IL-6, and IL-10 secretion, while at low concentrations only IL-6 secretion is observed. To obtain further evidence on in vivo conditions we investigated the development and structure of the protein corona of the nanoparticles. We demonstrated that the surface of TiO2 particles attract preferably secondary modified proteins which then induce cytokine secretion of macrophages. Our results indicate that concentration of corona covered TiO2 particles below 1μg/ml induce IL-6 secretion which is reported to be responsible for the development of autoimmune diseases as well as for the secretion of acute phase proteins.

Original languageEnglish (US)
Pages (from-to)275-282
Number of pages8
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume11
Issue number2
DOIs
StatePublished - Feb 1 2015

Keywords

  • Human macrophage
  • Immunology
  • Interleukin-6
  • Nanoparticles
  • Nanotoxicology
  • Protein corona
  • Titanium dioxide

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Molecular Medicine
  • Biomedical Engineering
  • Materials Science(all)
  • Pharmaceutical Science

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