TY - JOUR
T1 - Rapid identification of the invading microorganism
AU - Kaplan, Sheldon
AU - Feigin, R. D.
N1 - Funding Information:
"Assistant Professor of Pediatrics, Department of Pediatrics, Section of Infectious Diseases, Baylor College of Medicine, Houston, Texas tProfessor of Pediatrics, Baylor College of Medicine; Physician-in-Chief, Texas Children's Hospital, Houston, Texas Supported in part by Grant No. ROI-NS14292-03/04 from the National Institute of Neurological Disease and Stroke and the Myers-Black Mellon Enterprises Pediatric Infectious Diseases Research Fund.
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 1980
Y1 - 1980
N2 - In the past several years, many new laboratory procedures have been introduced for the rapid identification of specific microorganisms within various body fluids. These new laboratory procedures have proved to be effective when used in conjunction with, and not to the exclusion of, classic microbiologic techniques. A gram stain of any body fluid, such as cerebrospinal fluid or an aspirate from a septic joint, may provide very important diagnostic information rapidly and may allow preliminary identification of the particular organism responsible for the infection. However, the gram stain is subject to misinterpretation, particularly in inexperienced hands, and thus may provide unreliable information. In addition, prior treatment with antimicrobial agents may alter gram stain findings as well as culture results. With many of the newer laboratory techniques, prior antibiotic therapy does not alter results. Moreover, some of these techniques may provide useful prognostic information by additional semiquantitative modifications. We will describe some of these new laboratory techniques, summarize clinical studies utilizing them, and point out the advantages and disadvantages.
AB - In the past several years, many new laboratory procedures have been introduced for the rapid identification of specific microorganisms within various body fluids. These new laboratory procedures have proved to be effective when used in conjunction with, and not to the exclusion of, classic microbiologic techniques. A gram stain of any body fluid, such as cerebrospinal fluid or an aspirate from a septic joint, may provide very important diagnostic information rapidly and may allow preliminary identification of the particular organism responsible for the infection. However, the gram stain is subject to misinterpretation, particularly in inexperienced hands, and thus may provide unreliable information. In addition, prior treatment with antimicrobial agents may alter gram stain findings as well as culture results. With many of the newer laboratory techniques, prior antibiotic therapy does not alter results. Moreover, some of these techniques may provide useful prognostic information by additional semiquantitative modifications. We will describe some of these new laboratory techniques, summarize clinical studies utilizing them, and point out the advantages and disadvantages.
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U2 - 10.1016/S0031-3955(16)33926-8
DO - 10.1016/S0031-3955(16)33926-8
M3 - Article
C2 - 7005848
AN - SCOPUS:0019181972
VL - 27
SP - 783
EP - 803
JO - Pediatric Clinics of North America
JF - Pediatric Clinics of North America
SN - 0031-3955
IS - 4
ER -