Rapid identification of pathogens from positive blood cultures by multiplex polymerase chain reaction using the FilmArray system

Anne J. Blaschke; Caroline Heyrend; Carrie L. Byington; Mark A. Fisher; Elizabeth Barker; Nicholas F. Garrone; Stephanie A. Thatcher; Andrew T. Pavia; Trenda Barney; Garrison D. Alger; Judy A. Daly; Kirk M. Ririe; Irene Ota; Mark A. Poritz

doi:10.1016/j.diagmicrobio.2012.08.013

Rapid identification of pathogens from positive blood cultures by multiplex polymerase chain reaction using the FilmArray system

Anne J. Blaschke, Caroline Heyrend, Carrie L. Byington, Mark A. Fisher, Elizabeth Barker, Nicholas F. Garrone, Stephanie A. Thatcher, Andrew T. Pavia, Trenda Barney, Garrison D. Alger, Judy A. Daly, Kirk M. Ririe, Irene Ota, Mark A. Poritz

Academic Institute

Research output: Contribution to journal › Article › peer-review

178 Scopus citations

Abstract

Sepsis is a leading cause of death. Rapid and accurate identification of pathogens and antimicrobial resistance directly from blood culture could improve patient outcomes. The FilmArray® (FA; Idaho Technology, Salt Lake City, UT, USA) Blood Culture (BC) panel can identify >25 pathogens and 4 antibiotic resistance genes from positive blood cultures in 1 h. We compared a development version of the panel to conventional culture and susceptibility testing on 102 archived blood cultures from adults and children with bacteremia. Of 109 pathogens identified by culture, 95% were identified by FA. Among 111 prospectively collected blood cultures, the FA identified 84 (91%) of 92 pathogens covered by the panel. Among 25 Staphylococcus aureus and 21 Enterococcus species detected, FA identified all culture-proven methicillin-resistant S. aureus and vancomycin-resistant enterococci. The FA BC panel is an accurate method for the rapid identification of pathogens and resistance genes from blood culture.

Original language	English (US)
Pages (from-to)	349-355
Number of pages	7
Journal	Diagnostic Microbiology and Infectious Disease
Volume	74
Issue number	4
DOIs	https://doi.org/10.1016/j.diagmicrobio.2012.08.013
State	Published - Dec 2012

Keywords

Bacteremia
Blood culture
Bloodstream infection
FilmArray
Molecular diagnostics
Polymerase chain reaction
Sepsis

ASJC Scopus subject areas

Microbiology (medical)
Infectious Diseases

Access to Document

10.1016/j.diagmicrobio.2012.08.013

Cite this

Blaschke, A. J., Heyrend, C., Byington, C. L., Fisher, M. A., Barker, E., Garrone, N. F., Thatcher, S. A., Pavia, A. T., Barney, T., Alger, G. D., Daly, J. A., Ririe, K. M., Ota, I., & Poritz, M. A. (2012). Rapid identification of pathogens from positive blood cultures by multiplex polymerase chain reaction using the FilmArray system. Diagnostic Microbiology and Infectious Disease, 74(4), 349-355. https://doi.org/10.1016/j.diagmicrobio.2012.08.013

Blaschke, AJ, Heyrend, C, Byington, CL, Fisher, MA, Barker, E, Garrone, NF, Thatcher, SA, Pavia, AT, Barney, T, Alger, GD, Daly, JA, Ririe, KM, Ota, I & Poritz, MA 2012, 'Rapid identification of pathogens from positive blood cultures by multiplex polymerase chain reaction using the FilmArray system', Diagnostic Microbiology and Infectious Disease, vol. 74, no. 4, pp. 349-355. https://doi.org/10.1016/j.diagmicrobio.2012.08.013

@article{4e297eb40a374032a8696ee6f4464140,

title = "Rapid identification of pathogens from positive blood cultures by multiplex polymerase chain reaction using the FilmArray system",

abstract = "Sepsis is a leading cause of death. Rapid and accurate identification of pathogens and antimicrobial resistance directly from blood culture could improve patient outcomes. The FilmArray{\textregistered} (FA; Idaho Technology, Salt Lake City, UT, USA) Blood Culture (BC) panel can identify >25 pathogens and 4 antibiotic resistance genes from positive blood cultures in 1 h. We compared a development version of the panel to conventional culture and susceptibility testing on 102 archived blood cultures from adults and children with bacteremia. Of 109 pathogens identified by culture, 95\% were identified by FA. Among 111 prospectively collected blood cultures, the FA identified 84 (91\%) of 92 pathogens covered by the panel. Among 25 Staphylococcus aureus and 21 Enterococcus species detected, FA identified all culture-proven methicillin-resistant S. aureus and vancomycin-resistant enterococci. The FA BC panel is an accurate method for the rapid identification of pathogens and resistance genes from blood culture.",

keywords = "Bacteremia, Blood culture, Bloodstream infection, FilmArray, Molecular diagnostics, Polymerase chain reaction, Sepsis",

author = "Blaschke, \{Anne J.\} and Caroline Heyrend and Byington, \{Carrie L.\} and Fisher, \{Mark A.\} and Elizabeth Barker and Garrone, \{Nicholas F.\} and Thatcher, \{Stephanie A.\} and Pavia, \{Andrew T.\} and Trenda Barney and Alger, \{Garrison D.\} and Daly, \{Judy A.\} and Ririe, \{Kirk M.\} and Irene Ota and Poritz, \{Mark A.\}",

year = "2012",

month = dec,

doi = "10.1016/j.diagmicrobio.2012.08.013",

language = "English (US)",

volume = "74",

pages = "349--355",

journal = "Diagnostic Microbiology and Infectious Disease",

issn = "0732-8893",

publisher = "Elsevier",

number = "4",

}

TY - JOUR

T1 - Rapid identification of pathogens from positive blood cultures by multiplex polymerase chain reaction using the FilmArray system

AU - Blaschke, Anne J.

AU - Heyrend, Caroline

AU - Byington, Carrie L.

AU - Fisher, Mark A.

AU - Barker, Elizabeth

AU - Garrone, Nicholas F.

AU - Thatcher, Stephanie A.

AU - Pavia, Andrew T.

AU - Barney, Trenda

AU - Alger, Garrison D.

AU - Daly, Judy A.

AU - Ririe, Kirk M.

AU - Ota, Irene

AU - Poritz, Mark A.

PY - 2012/12

Y1 - 2012/12

N2 - Sepsis is a leading cause of death. Rapid and accurate identification of pathogens and antimicrobial resistance directly from blood culture could improve patient outcomes. The FilmArray® (FA; Idaho Technology, Salt Lake City, UT, USA) Blood Culture (BC) panel can identify >25 pathogens and 4 antibiotic resistance genes from positive blood cultures in 1 h. We compared a development version of the panel to conventional culture and susceptibility testing on 102 archived blood cultures from adults and children with bacteremia. Of 109 pathogens identified by culture, 95% were identified by FA. Among 111 prospectively collected blood cultures, the FA identified 84 (91%) of 92 pathogens covered by the panel. Among 25 Staphylococcus aureus and 21 Enterococcus species detected, FA identified all culture-proven methicillin-resistant S. aureus and vancomycin-resistant enterococci. The FA BC panel is an accurate method for the rapid identification of pathogens and resistance genes from blood culture.

AB - Sepsis is a leading cause of death. Rapid and accurate identification of pathogens and antimicrobial resistance directly from blood culture could improve patient outcomes. The FilmArray® (FA; Idaho Technology, Salt Lake City, UT, USA) Blood Culture (BC) panel can identify >25 pathogens and 4 antibiotic resistance genes from positive blood cultures in 1 h. We compared a development version of the panel to conventional culture and susceptibility testing on 102 archived blood cultures from adults and children with bacteremia. Of 109 pathogens identified by culture, 95% were identified by FA. Among 111 prospectively collected blood cultures, the FA identified 84 (91%) of 92 pathogens covered by the panel. Among 25 Staphylococcus aureus and 21 Enterococcus species detected, FA identified all culture-proven methicillin-resistant S. aureus and vancomycin-resistant enterococci. The FA BC panel is an accurate method for the rapid identification of pathogens and resistance genes from blood culture.

KW - Bacteremia

KW - Blood culture

KW - Bloodstream infection

KW - FilmArray

KW - Molecular diagnostics

KW - Polymerase chain reaction

KW - Sepsis

UR - https://www.scopus.com/pages/publications/84869085496

UR - https://www.scopus.com/inward/citedby.url?scp=84869085496&partnerID=8YFLogxK

U2 - 10.1016/j.diagmicrobio.2012.08.013

DO - 10.1016/j.diagmicrobio.2012.08.013

M3 - Article

C2 - 22999332

AN - SCOPUS:84869085496

SN - 0732-8893

VL - 74

SP - 349

EP - 355

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

IS - 4

ER -

Rapid identification of pathogens from positive blood cultures by multiplex polymerase chain reaction using the FilmArray system

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this