Rapid identification of pathogens from positive blood cultures by multiplex polymerase chain reaction using the FilmArray system

Anne J. Blaschke, Caroline Heyrend, Carrie L. Byington, Mark A. Fisher, Elizabeth Barker, Nicholas F. Garrone, Stephanie A. Thatcher, Andrew T. Pavia, Trenda Barney, Garrison D. Alger, Judy A. Daly, Kirk M. Ririe, Irene Ota, Mark A. Poritz

Research output: Contribution to journalArticle

124 Scopus citations

Abstract

Sepsis is a leading cause of death. Rapid and accurate identification of pathogens and antimicrobial resistance directly from blood culture could improve patient outcomes. The FilmArray® (FA; Idaho Technology, Salt Lake City, UT, USA) Blood Culture (BC) panel can identify >25 pathogens and 4 antibiotic resistance genes from positive blood cultures in 1 h. We compared a development version of the panel to conventional culture and susceptibility testing on 102 archived blood cultures from adults and children with bacteremia. Of 109 pathogens identified by culture, 95% were identified by FA. Among 111 prospectively collected blood cultures, the FA identified 84 (91%) of 92 pathogens covered by the panel. Among 25 Staphylococcus aureus and 21 Enterococcus species detected, FA identified all culture-proven methicillin-resistant S. aureus and vancomycin-resistant enterococci. The FA BC panel is an accurate method for the rapid identification of pathogens and resistance genes from blood culture.

Original languageEnglish (US)
Pages (from-to)349-355
Number of pages7
JournalDiagnostic Microbiology and Infectious Disease
Volume74
Issue number4
DOIs
StatePublished - Dec 2012

Keywords

  • Bacteremia
  • Blood culture
  • Bloodstream infection
  • FilmArray
  • Molecular diagnostics
  • Polymerase chain reaction
  • Sepsis

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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