Rapid identification of Enterococcus hirae and Enterococcus durans by PCR and detection of a homologue of the E. hirae mur-2 gene in E. durans

Cesar A. Arias, Beatriz Robredo, Kavindra Singh, Carmen Torres, Diana Panesso, Barbara E. Murray

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

During an evaluation of PCR for identification of isolates of Enterococcus hirae, a homologue with 82% identity to E. hirae mur-2 was identified in Enterococcus durans and was named mur-2ed. PCR using primers for two genes (copY and murG) of E. hirae strains showed amplification with E. hirae strains only. PCR (under high-stringency conditions) with primers for the mur-2ed gene gave the expected amplification product only with E. durans strains. A combination of murG and mur-2ed primers in a multiplex PCR assay differentiated E. hirae from E. durans in all cases. PCR using these primers appears to be a rapid alternative for identification of E. hirae and E. durans isolates.

Original languageEnglish (US)
Pages (from-to)1567-1570
Number of pages4
JournalJournal of Clinical Microbiology
Volume44
Issue number4
DOIs
StatePublished - Apr 2006

ASJC Scopus subject areas

  • Microbiology (medical)

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