Rapid biogenesis and sensitization of secretory lysosomes in NK cells mediated by target-cell recognition

Dongfang Liu, Liang Xu, Fan Yang, Dongdong Li, Feili Gong, Tao Xu

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

Natural killer (NK) cells play important roles in defense against tumor, viral infection, and cell-mediated xenograft rejection through secretion of secretory lysosomes. In this study, we used high time-resolution membrane capacitance measurement and fluorescence-imaging techniques to study the biogenesis and exocytosis of secretory lysosomes in a human NK cell line. We demonstrated a high-affinity Ca 2+-dependent exocytosis of secretory lysosomes, which is sensitized further after target-cell stimulation. Our data also suggest an unusual rapid and dramatic de novo formation of secretory lysosomes after target-cell recognition. The rapid biogenesis of secretory lysosomes was blocked by specific protein kinase C inhibitor but not by brefeldin A. We propose that target-cell recognition triggers rapid biogenesis and sensitization of secretory lysosomes in NK cells through activation of PKC.

Original languageEnglish (US)
Pages (from-to)123-127
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume102
Issue number1
DOIs
StatePublished - Jan 4 2005

Keywords

  • Calcium
  • Defense
  • Exocytosis
  • Immune
  • Lytic granules

ASJC Scopus subject areas

  • General

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