Rapamycin-induced enhancement of vaccine efficacy in mice

Chinnaswamy Jagannath, Pearl Bakhru

Research output: Chapter in Book/Report/Conference proceedingChapter

27 Scopus citations

Abstract

Th1 immunity protects against tuberculosis infection in mice and humans. The widely used BCG vaccine primes CD4 and CD8 T cells through signaling mechanisms from dendritic cells and macrophages. The latter express MHC-II and MHC-I molecules through which peptides from BCG vaccine are presented to CD4 and CD8 T cells, respectively. Since BCG sequesters within a phagosome that does not fuse with lysosomes, generation of peptides within antigen-presenting cells infected with BCG occurs with reduced efficiency. We demonstrate that activation of DCs containing BCG vaccine with rapamycin leads to an enhanced ability of DC vaccines to immunize mice against tuberculosis. Coadministration of rapamycin with BCG vaccine also enhanced Th1 immunity. We propose that rapamycin-mediated increase in Th1 responses offers novel models to study mTOR-mediated regulation of immunity.

Original languageEnglish (US)
Title of host publicationmTOR
Subtitle of host publicationMethods and Protocols
EditorsThomas Weichhart
Pages295-303
Number of pages9
DOIs
StatePublished - 2012
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume821
ISSN (Print)1064-3745

Keywords

  • BCG vaccine
  • CD4
  • CD8 T cells
  • Mouse
  • Mycobacterium tuberculosis
  • Rapamycin
  • Th1 immunity
  • mTOR

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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