Radioimmunoassay of rat apolipoprotein A-IV

William L. Crump, Bette C. Sherrill, Antonio Gotto, Giancarlo Ghiselli

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

We have developed a specific and sensitive radioimmunoassay for rat apolipoprotein A-IV (apoA-IV). The protocol includes treatment of the samples for 1 h at 60°C with 0.7% Tween 20. Under these conditions, linear logit-log plots have been obtained for apoA-IV in lymph and plasma lipoprotein fractions as well as for purified apoA-IV. The sensitivity of the assay is to 20 ng. Absolute mass values obtained with the assay were validated by comparison with values obtained with an independent method of colorimetric reading of apoA-IV separated by polyacrylamide gel electrophoresis from plasma high density lipoproteins. The concentration of apoA-IV in fasting plasma averaged 10.2 mg/dl and in the mesenteric duct lymph 15.8 and 12.6 mg/dl during the fasting and the fat absorption states, respectively.

Original languageEnglish (US)
Pages (from-to)96-100
Number of pages5
JournalAnalytical Biochemistry
Volume159
Issue number1
DOIs
StatePublished - Nov 15 1986

Keywords

  • apolipoprotein A-IV
  • gel electrophoresis
  • plasma lipoproteins
  • radioimmune assays
  • thoracic duct lymph lipoproteins
  • Tween 20

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

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