Radioimmunoassay of rat apolipoprotein A-IV

William L. Crump; Bette C. Sherrill; Antonio Gotto; Giancarlo Ghiselli

doi:10.1016/0003-2697(86)90312-X

Radioimmunoassay of rat apolipoprotein A-IV

William L. Crump, Bette C. Sherrill, Antonio Gotto, Giancarlo Ghiselli

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Abstract

We have developed a specific and sensitive radioimmunoassay for rat apolipoprotein A-IV (apoA-IV). The protocol includes treatment of the samples for 1 h at 60°C with 0.7% Tween 20. Under these conditions, linear logit-log plots have been obtained for apoA-IV in lymph and plasma lipoprotein fractions as well as for purified apoA-IV. The sensitivity of the assay is to 20 ng. Absolute mass values obtained with the assay were validated by comparison with values obtained with an independent method of colorimetric reading of apoA-IV separated by polyacrylamide gel electrophoresis from plasma high density lipoproteins. The concentration of apoA-IV in fasting plasma averaged 10.2 mg/dl and in the mesenteric duct lymph 15.8 and 12.6 mg/dl during the fasting and the fat absorption states, respectively.

Original language	English (US)
Pages (from-to)	96-100
Number of pages	5
Journal	Analytical Biochemistry
Volume	159
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(86)90312-X
State	Published - Nov 15 1986

Keywords

apolipoprotein A-IV
gel electrophoresis
plasma lipoproteins
radioimmune assays
thoracic duct lymph lipoproteins
Tween 20

ASJC Scopus subject areas

Molecular Biology
Biophysics
Biochemistry

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10.1016/0003-2697(86)90312-X

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title = "Radioimmunoassay of rat apolipoprotein A-IV",

abstract = "We have developed a specific and sensitive radioimmunoassay for rat apolipoprotein A-IV (apoA-IV). The protocol includes treatment of the samples for 1 h at 60°C with 0.7% Tween 20. Under these conditions, linear logit-log plots have been obtained for apoA-IV in lymph and plasma lipoprotein fractions as well as for purified apoA-IV. The sensitivity of the assay is to 20 ng. Absolute mass values obtained with the assay were validated by comparison with values obtained with an independent method of colorimetric reading of apoA-IV separated by polyacrylamide gel electrophoresis from plasma high density lipoproteins. The concentration of apoA-IV in fasting plasma averaged 10.2 mg/dl and in the mesenteric duct lymph 15.8 and 12.6 mg/dl during the fasting and the fat absorption states, respectively.",

keywords = "apolipoprotein A-IV, gel electrophoresis, plasma lipoproteins, radioimmune assays, thoracic duct lymph lipoproteins, Tween 20",

author = "Crump, {William L.} and Sherrill, {Bette C.} and Antonio Gotto and Giancarlo Ghiselli",

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T1 - Radioimmunoassay of rat apolipoprotein A-IV

AU - Crump, William L.

AU - Sherrill, Bette C.

AU - Gotto, Antonio

AU - Ghiselli, Giancarlo

PY - 1986/11/15

Y1 - 1986/11/15

N2 - We have developed a specific and sensitive radioimmunoassay for rat apolipoprotein A-IV (apoA-IV). The protocol includes treatment of the samples for 1 h at 60°C with 0.7% Tween 20. Under these conditions, linear logit-log plots have been obtained for apoA-IV in lymph and plasma lipoprotein fractions as well as for purified apoA-IV. The sensitivity of the assay is to 20 ng. Absolute mass values obtained with the assay were validated by comparison with values obtained with an independent method of colorimetric reading of apoA-IV separated by polyacrylamide gel electrophoresis from plasma high density lipoproteins. The concentration of apoA-IV in fasting plasma averaged 10.2 mg/dl and in the mesenteric duct lymph 15.8 and 12.6 mg/dl during the fasting and the fat absorption states, respectively.

AB - We have developed a specific and sensitive radioimmunoassay for rat apolipoprotein A-IV (apoA-IV). The protocol includes treatment of the samples for 1 h at 60°C with 0.7% Tween 20. Under these conditions, linear logit-log plots have been obtained for apoA-IV in lymph and plasma lipoprotein fractions as well as for purified apoA-IV. The sensitivity of the assay is to 20 ng. Absolute mass values obtained with the assay were validated by comparison with values obtained with an independent method of colorimetric reading of apoA-IV separated by polyacrylamide gel electrophoresis from plasma high density lipoproteins. The concentration of apoA-IV in fasting plasma averaged 10.2 mg/dl and in the mesenteric duct lymph 15.8 and 12.6 mg/dl during the fasting and the fat absorption states, respectively.

KW - apolipoprotein A-IV

KW - gel electrophoresis

KW - plasma lipoproteins

KW - radioimmune assays

KW - thoracic duct lymph lipoproteins

KW - Tween 20

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JO - Analytical Biochemistry

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Radioimmunoassay of rat apolipoprotein A-IV

Abstract

Keywords

ASJC Scopus subject areas

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