Quantitative synaptic vesicle imaging for evaluating neuron activities in neurodegenerative diseases

Jing Fan, Xiaofeng Xia, Jennifer Dy, Stephen Wong

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Synaptic vesicle dynamics play an important role in studying neuronal and synaptic activities of neurodegenerative diseases ranging from epidemic Alzheimer's disease to rare Rett syndrome. To obtain significant statistical power in such studies, we developed a high content analysis (HCA) pipeline to visualize the vesicle dynamics and characterize the neuronal synaptic activities in a large population of neurons. Our experiments on hippocampal neuron assays showed that the proposed HCA system can automatically detect vesicles and quantify their dynamics for evaluating neuron activities. The availability of such an automated system would open up a vista to investigate synaptic neuropathology and identify candidate therapeutics of neurodegeneration.

Original languageEnglish (US)
Title of host publicationConference Record of the 45th Asilomar Conference on Signals, Systems and Computers, ASILOMAR 2011
Pages421-425
Number of pages5
DOIs
StatePublished - 2011
Event45th Asilomar Conference on Signals, Systems and Computers, ASILOMAR 2011 - Pacific Grove, CA, United States
Duration: Nov 6 2011Nov 9 2011

Publication series

NameConference Record - Asilomar Conference on Signals, Systems and Computers
ISSN (Print)1058-6393

Other

Other45th Asilomar Conference on Signals, Systems and Computers, ASILOMAR 2011
Country/TerritoryUnited States
CityPacific Grove, CA
Period11/6/1111/9/11

Keywords

  • detection and quantification
  • high throughput study
  • neurodegenerative disease
  • neuron activity
  • synaptic vesicle

ASJC Scopus subject areas

  • Computer Networks and Communications
  • Signal Processing

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