Quantitative study of aluminum binding to human serum albumin and transferrin by a chelex competitive binding assay

Roger L. Bertholf; Michael R. Wills; John Savory

doi:10.1016/0006-291X(84)91385-8

Quantitative study of aluminum binding to human serum albumin and transferrin by a chelex competitive binding assay

Roger L. Bertholf, Michael R. Wills, John Savory

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Abstract

Binding of aluminum to human serum albumin and transferrin was investigated using a competitive binding assay incorporating a cation exchange resin, chelex. Both albumin and transferrin were found to produce linear Scatchard plots of aluminum binding data over the aluminum and protein concentration ranges found in humans. Binding constants measured for albumin and transferrin were 1.96 and 0.515 μM, respectively.

Original language	English (US)
Pages (from-to)	1020-1024
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	125
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(84)91385-8
State	Published - Dec 28 1984

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(84)91385-8

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title = "Quantitative study of aluminum binding to human serum albumin and transferrin by a chelex competitive binding assay",

abstract = "Binding of aluminum to human serum albumin and transferrin was investigated using a competitive binding assay incorporating a cation exchange resin, chelex. Both albumin and transferrin were found to produce linear Scatchard plots of aluminum binding data over the aluminum and protein concentration ranges found in humans. Binding constants measured for albumin and transferrin were 1.96 and 0.515 μM, respectively.",

author = "Bertholf, \{Roger L.\} and Wills, \{Michael R.\} and John Savory",

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T1 - Quantitative study of aluminum binding to human serum albumin and transferrin by a chelex competitive binding assay

AU - Bertholf, Roger L.

AU - Wills, Michael R.

AU - Savory, John

PY - 1984/12/28

Y1 - 1984/12/28

N2 - Binding of aluminum to human serum albumin and transferrin was investigated using a competitive binding assay incorporating a cation exchange resin, chelex. Both albumin and transferrin were found to produce linear Scatchard plots of aluminum binding data over the aluminum and protein concentration ranges found in humans. Binding constants measured for albumin and transferrin were 1.96 and 0.515 μM, respectively.

AB - Binding of aluminum to human serum albumin and transferrin was investigated using a competitive binding assay incorporating a cation exchange resin, chelex. Both albumin and transferrin were found to produce linear Scatchard plots of aluminum binding data over the aluminum and protein concentration ranges found in humans. Binding constants measured for albumin and transferrin were 1.96 and 0.515 μM, respectively.

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U2 - 10.1016/0006-291X(84)91385-8

DO - 10.1016/0006-291X(84)91385-8

M3 - Article

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AN - SCOPUS:0021673838

SN - 0006-291X

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JO - Biochemical and Biophysical Research Communications

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IS - 3

ER -

Quantitative study of aluminum binding to human serum albumin and transferrin by a chelex competitive binding assay

Abstract

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