Quantitative study of aluminum binding to human serum albumin and transferrin by a chelex competitive binding assay

Roger L. Bertholf, Michael R. Wills, John Savory

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Binding of aluminum to human serum albumin and transferrin was investigated using a competitive binding assay incorporating a cation exchange resin, chelex. Both albumin and transferrin were found to produce linear Scatchard plots of aluminum binding data over the aluminum and protein concentration ranges found in humans. Binding constants measured for albumin and transferrin were 1.96 and 0.515 μM, respectively.

Original languageEnglish (US)
Pages (from-to)1020-1024
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume125
Issue number3
DOIs
StatePublished - Dec 28 1984

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Quantitative study of aluminum binding to human serum albumin and transferrin by a chelex competitive binding assay'. Together they form a unique fingerprint.

Cite this