Quantitative structure-activity relationships: Analysis of interactions of 2,3,7,8-tetrachlorodibenzo-p-dioxin and 2-substituted analogues with rat, mouse, guinea pig, and hamster cytosolic receptor

M. Romkes, J. Piskorska-Pliszczynska, B. Keys, S. Safe, T. Fujita

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

The competitive receptor binding affinities of thirteen 2-substituted 3,7,8-trichlorodibenzo-p-dioxins to hepatic cytosol from rat, mouse, guinea pig, and hamster were determined with [3H]-2,3,7,8-tetrachlorodibenzo-p-dioxin as the radioligand. Multiple parameter linear regression analysis of the binding data from the four species gave the following equations: pEC50 (rat) = 7.196 + 0.600 π - 0.255 ΔE(s) - 1.683 HB, pEC50 (mouse) = 6.365 + 1.641 π + 1.206 σ°, pEC50 (hamster) = 7.416 + 1.026 π + 0.509ΔE(s) + 0.748 σ°, pEC50 (guinea pig) = 6.892 + 1.035 π where π, ΔE(s), HB, σ°, and V(w) are physicochemical parameters for substitutent lipophilicity, steric effect, hydrogen bonding capacity, electronegativity, and van der Waals volume (relative to H), respectively. These equations demonstrate that there are important species differences in the receptor protein binding site interactions with the substituted analogues. These data, coupled with the known species differences in the molecular properties of the receptor proteins, are evidence for a heterologous nature of the receptor between mammalian species. Multiple parameter linear regression analysis of the relative aryl hydrocarbon hydroxylase (AHH) induction potencies of these analogues in rat hepatoma H-4-II E-cells in culture gave the following equation. The correlation pEC50 (AHH induction) = 3.208 = 0.950 pEC50 (rat binding) - 0.955 ΔB5 between receptor binding and AHH induction was dependent on a steric parameter (ΔB5, STERIMOL) and the results suggest that an additional substituent-dependent process (e.g., an activation step) may be required after initial ligand-receptor binding for the ultimate expression of the receptor-mediated response (i.e., AHH induction).

Original languageEnglish (US)
Pages (from-to)5108-5111
Number of pages4
JournalCancer research
Volume47
Issue number19
StatePublished - 1987

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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