TY - JOUR
T1 - Quantitative gel-electrophoretic determination of serum amylase isoenzyme distributions
AU - Gillard, B. K.
PY - 1979
Y1 - 1979
N2 - The author reports a direct, sensitive, quantitative method for determining serum amylase isoenzyme activity with commercially available reagents. Day-to-day reproducibility (CV) was 3-4% for the isoenzymes in normal serum; within-run precision was 8, 3, and 2% for low, normal, and high isoenzyme activities. Amylase isoenzymes, separated into the pancreatic and salivary types by electrophoresis in polyacrylamide gel, are then quantified by directly incubating the gels in soluble-starch solution, staining with iodine, and densitometry. The proportion of pancreatic isoenzyme (47 normal sera) was 43 ± 8% (mean ± SD). Isoenzyme activities as low as 2% of normal can be measured accurately in 10 μL of serum. The reproducibility, precision, and sensitivity indicate that the method is applicable to differential diagnosis of hyperamylasemia or hypoamylasemia, and is suited for monitoring the subtle changes in serum anylase isoenzyme distribution that may accompany disease progression or therapy.
AB - The author reports a direct, sensitive, quantitative method for determining serum amylase isoenzyme activity with commercially available reagents. Day-to-day reproducibility (CV) was 3-4% for the isoenzymes in normal serum; within-run precision was 8, 3, and 2% for low, normal, and high isoenzyme activities. Amylase isoenzymes, separated into the pancreatic and salivary types by electrophoresis in polyacrylamide gel, are then quantified by directly incubating the gels in soluble-starch solution, staining with iodine, and densitometry. The proportion of pancreatic isoenzyme (47 normal sera) was 43 ± 8% (mean ± SD). Isoenzyme activities as low as 2% of normal can be measured accurately in 10 μL of serum. The reproducibility, precision, and sensitivity indicate that the method is applicable to differential diagnosis of hyperamylasemia or hypoamylasemia, and is suited for monitoring the subtle changes in serum anylase isoenzyme distribution that may accompany disease progression or therapy.
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U2 - 10.1093/clinchem/25.11.1919
DO - 10.1093/clinchem/25.11.1919
M3 - Article
C2 - 498502
AN - SCOPUS:0018642949
VL - 25
SP - 1919
EP - 1923
JO - Clinical Chemistry
JF - Clinical Chemistry
SN - 0009-9147
IS - 11
ER -