Quantitative endothelial biomicroscopy

J. T. Holladay, J. E. Bishop, Thomas Prager

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


Twenty patients were evaluated by quantitative endothelial biomicroscopy and the resulting endothelial cell densities compared with those from a specular microscope. Estimated cell densities by this technique, when compared with the specular microscope, demonstrated a Pearson correlation coefficient of +0.977, an average error of -7% and an absolute error of 12%. There were no errors greater than 26% except for one extremely low cell density of 318 cells/mm2 for which the estimate was 476 cells/mm2. This rapid, inexpensive technique requires counting the number of endothelial cells seen across the horizontal diameter of the 0.2-mm projected spot beam of a standard biomicroscope. From this count and the known spot size the endothelial cell density may be accurately calculated. A simplified four-step technique for performing specular microscopy using only the biomicroscope and a method for accurately measuring the size of the projected slit lamp spot beam are explained.

Original languageEnglish (US)
Pages (from-to)33-40
Number of pages8
JournalOphthalmic Surgery
Issue number1
StatePublished - Jan 1 1983

ASJC Scopus subject areas

  • Ophthalmology


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