Quantitative analysis of Gd@C82(OH)22 and cisplatin uptake in single cells by inductively coupled plasma mass spectrometry

Ling Na Zheng; Meng Wang; Lei Chao Zhao; Bao Yun Sun; Bing Wang; Han Qing Chen; Yu Liang Zhao; Zhi Fang Chai; Wei Yue Feng

doi:10.1007/s00216-014-8422-3

Quantitative analysis of Gd@C₈₂(OH)₂₂ and cisplatin uptake in single cells by inductively coupled plasma mass spectrometry

Ling Na Zheng, Meng Wang, Lei Chao Zhao, Bao Yun Sun, Bing Wang, Han Qing Chen, Yu Liang Zhao, Zhi Fang Chai, Wei Yue Feng

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

Abstract Cisplatin is a commonly used chemotherapeutic drug in cancer treatment, whereas Gd@C₈₂(OH)₂₂ is a new nanomaterial anti-tumor agent. In this study, we determined intracellular Gd@C₈₂(OH)₂₂ and cisplatin after treatment of Hela and 16HBE cells by single cell inductively coupled plasma-mass spectrometry (SC-ICP-MS), which could provide quantitative information at a single-cell level. The cell digestion method validated the accuracy of the SC-ICP-MS. The concentrations of Gd@C₈₂(OH)₂₂ and cisplatin in cells at different exposure times and doses were studied. The SC-ICP-MS is a promising complement to available methods for single cell analysis and is anticipated to be applied further to biomedical research.

Original language	English (US)
Article number	8422
Pages (from-to)	2383-2391
Number of pages	9
Journal	Analytical and Bioanalytical Chemistry
Volume	407
Issue number	9
DOIs	https://doi.org/10.1007/s00216-014-8422-3
State	Published - Mar 19 2015

Keywords

Cisplatin
Gd@C(OH)
ICP-MS
Quantitative analysis
Single cell

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry

Access to Document

10.1007/s00216-014-8422-3

Cite this

@article{b68b571b71264213a43fdfe976261c4c,

title = "Quantitative analysis of Gd@C82(OH)22 and cisplatin uptake in single cells by inductively coupled plasma mass spectrometry",

abstract = "Abstract Cisplatin is a commonly used chemotherapeutic drug in cancer treatment, whereas Gd@C82(OH)22 is a new nanomaterial anti-tumor agent. In this study, we determined intracellular Gd@C82(OH)22 and cisplatin after treatment of Hela and 16HBE cells by single cell inductively coupled plasma-mass spectrometry (SC-ICP-MS), which could provide quantitative information at a single-cell level. The cell digestion method validated the accuracy of the SC-ICP-MS. The concentrations of Gd@C82(OH)22 and cisplatin in cells at different exposure times and doses were studied. The SC-ICP-MS is a promising complement to available methods for single cell analysis and is anticipated to be applied further to biomedical research.",

keywords = "Cisplatin, Gd@C(OH), ICP-MS, Quantitative analysis, Single cell",

author = "Zheng, {Ling Na} and Meng Wang and Zhao, {Lei Chao} and Sun, {Bao Yun} and Bing Wang and Chen, {Han Qing} and Zhao, {Yu Liang} and Chai, {Zhi Fang} and Feng, {Wei Yue}",

note = "Funding Information: This work was supported by the National Basic Research Program (973 Program: 2011CB933403) and the National Natural Science Foundation of China (21175136, 11275214, and 11375211). Publisher Copyright: {\textcopyright} 2015 Springer-Verlag Berlin Heidelberg.",

year = "2015",

month = mar,

day = "19",

doi = "10.1007/s00216-014-8422-3",

language = "English (US)",

volume = "407",

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TY - JOUR

T1 - Quantitative analysis of Gd@C82(OH)22 and cisplatin uptake in single cells by inductively coupled plasma mass spectrometry

AU - Zheng, Ling Na

AU - Wang, Meng

AU - Zhao, Lei Chao

AU - Sun, Bao Yun

AU - Wang, Bing

AU - Chen, Han Qing

AU - Zhao, Yu Liang

AU - Chai, Zhi Fang

AU - Feng, Wei Yue

N1 - Funding Information: This work was supported by the National Basic Research Program (973 Program: 2011CB933403) and the National Natural Science Foundation of China (21175136, 11275214, and 11375211). Publisher Copyright: © 2015 Springer-Verlag Berlin Heidelberg.

PY - 2015/3/19

Y1 - 2015/3/19

N2 - Abstract Cisplatin is a commonly used chemotherapeutic drug in cancer treatment, whereas Gd@C82(OH)22 is a new nanomaterial anti-tumor agent. In this study, we determined intracellular Gd@C82(OH)22 and cisplatin after treatment of Hela and 16HBE cells by single cell inductively coupled plasma-mass spectrometry (SC-ICP-MS), which could provide quantitative information at a single-cell level. The cell digestion method validated the accuracy of the SC-ICP-MS. The concentrations of Gd@C82(OH)22 and cisplatin in cells at different exposure times and doses were studied. The SC-ICP-MS is a promising complement to available methods for single cell analysis and is anticipated to be applied further to biomedical research.

AB - Abstract Cisplatin is a commonly used chemotherapeutic drug in cancer treatment, whereas Gd@C82(OH)22 is a new nanomaterial anti-tumor agent. In this study, we determined intracellular Gd@C82(OH)22 and cisplatin after treatment of Hela and 16HBE cells by single cell inductively coupled plasma-mass spectrometry (SC-ICP-MS), which could provide quantitative information at a single-cell level. The cell digestion method validated the accuracy of the SC-ICP-MS. The concentrations of Gd@C82(OH)22 and cisplatin in cells at different exposure times and doses were studied. The SC-ICP-MS is a promising complement to available methods for single cell analysis and is anticipated to be applied further to biomedical research.

KW - Cisplatin

KW - Gd@C(OH)

KW - ICP-MS

KW - Quantitative analysis

KW - Single cell

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