Quantitative analysis of Gd@C82(OH)22 and cisplatin uptake in single cells by inductively coupled plasma mass spectrometry

Ling Na Zheng, Meng Wang, Lei Chao Zhao, Bao Yun Sun, Bing Wang, Han Qing Chen, Yu Liang Zhao, Zhi Fang Chai, Wei Yue Feng

    Research output: Contribution to journalArticlepeer-review

    47 Scopus citations

    Abstract

    Abstract Cisplatin is a commonly used chemotherapeutic drug in cancer treatment, whereas Gd@C82(OH)22 is a new nanomaterial anti-tumor agent. In this study, we determined intracellular Gd@C82(OH)22 and cisplatin after treatment of Hela and 16HBE cells by single cell inductively coupled plasma-mass spectrometry (SC-ICP-MS), which could provide quantitative information at a single-cell level. The cell digestion method validated the accuracy of the SC-ICP-MS. The concentrations of Gd@C82(OH)22 and cisplatin in cells at different exposure times and doses were studied. The SC-ICP-MS is a promising complement to available methods for single cell analysis and is anticipated to be applied further to biomedical research.

    Original languageEnglish (US)
    Article number8422
    Pages (from-to)2383-2391
    Number of pages9
    JournalAnalytical and Bioanalytical Chemistry
    Volume407
    Issue number9
    DOIs
    StatePublished - Mar 19 2015

    Keywords

    • Cisplatin
    • Gd@C(OH)
    • ICP-MS
    • Quantitative analysis
    • Single cell

    ASJC Scopus subject areas

    • Analytical Chemistry
    • Biochemistry

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