Abstract
Abstract Cisplatin is a commonly used chemotherapeutic drug in cancer treatment, whereas Gd@C82(OH)22 is a new nanomaterial anti-tumor agent. In this study, we determined intracellular Gd@C82(OH)22 and cisplatin after treatment of Hela and 16HBE cells by single cell inductively coupled plasma-mass spectrometry (SC-ICP-MS), which could provide quantitative information at a single-cell level. The cell digestion method validated the accuracy of the SC-ICP-MS. The concentrations of Gd@C82(OH)22 and cisplatin in cells at different exposure times and doses were studied. The SC-ICP-MS is a promising complement to available methods for single cell analysis and is anticipated to be applied further to biomedical research.
Original language | English (US) |
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Article number | 8422 |
Pages (from-to) | 2383-2391 |
Number of pages | 9 |
Journal | Analytical and Bioanalytical Chemistry |
Volume | 407 |
Issue number | 9 |
DOIs | |
State | Published - Mar 19 2015 |
Keywords
- Cisplatin
- Gd@C(OH)
- ICP-MS
- Quantitative analysis
- Single cell
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry