Quantitative analysis of Gd@C82(OH)22 and cisplatin uptake in single cells by inductively coupled plasma mass spectrometry

Ling Na Zheng, Meng Wang, Lei Chao Zhao, Bao Yun Sun, Bing Wang, Han Qing Chen, Yu Liang Zhao, Zhi Fang Chai, Wei Yue Feng

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Abstract Cisplatin is a commonly used chemotherapeutic drug in cancer treatment, whereas Gd@C82(OH)22 is a new nanomaterial anti-tumor agent. In this study, we determined intracellular Gd@C82(OH)22 and cisplatin after treatment of Hela and 16HBE cells by single cell inductively coupled plasma-mass spectrometry (SC-ICP-MS), which could provide quantitative information at a single-cell level. The cell digestion method validated the accuracy of the SC-ICP-MS. The concentrations of Gd@C82(OH)22 and cisplatin in cells at different exposure times and doses were studied. The SC-ICP-MS is a promising complement to available methods for single cell analysis and is anticipated to be applied further to biomedical research.

Original languageEnglish (US)
Article number8422
Pages (from-to)2383-2391
Number of pages9
JournalAnalytical and Bioanalytical Chemistry
Volume407
Issue number9
DOIs
StatePublished - Mar 19 2015

Keywords

  • Cisplatin
  • Gd@C(OH)
  • ICP-MS
  • Quantitative analysis
  • Single cell

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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