Quantitation of Hymenoxon and Related Sesquiterpene Lactones

Hyeong L. Kim, Stephen H. Safe, Millard C. Calhoun

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

A rapid and reproducible method for the quantitation of hymenoxon and related sesquiterpene lactones has been developed using reversed-phase HPLC. The methanol extract of a dried and ground plant sample was analyzed on an RP-8 column eluted with methanol-water (1:1, v/v) and monitored at 235 nm. The hymenoxon content of the above ground whole plant of Hymenoxys odorata ranged from 0.21 to 1.27% according to the site, season, and year of collection. Helenalin and mexicanin E were concentrated in the flowering heads (8.95 and 4.66%, respectively) of Helenium microcephalum while tenulin was concentrated in the leaves (6.14%) of Helenium amarum. The detection limit was 25 ng for helenalin and mexicanin E and 75 ng for hymenoxon and tenulin. The recovery rate of hymenoxon was 102.5 ± 4.5%.

Original languageEnglish (US)
Pages (from-to)891-895
Number of pages5
JournalJournal of Agricultural and Food Chemistry
Volume35
Issue number6
DOIs
StatePublished - Nov 1 1987

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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