Abstract
A rapid and reproducible method for the quantitation of hymenoxon and related sesquiterpene lactones has been developed using reversed-phase HPLC. The methanol extract of a dried and ground plant sample was analyzed on an RP-8 column eluted with methanol-water (1:1, v/v) and monitored at 235 nm. The hymenoxon content of the above ground whole plant of Hymenoxys odorata ranged from 0.21 to 1.27% according to the site, season, and year of collection. Helenalin and mexicanin E were concentrated in the flowering heads (8.95 and 4.66%, respectively) of Helenium microcephalum while tenulin was concentrated in the leaves (6.14%) of Helenium amarum. The detection limit was 25 ng for helenalin and mexicanin E and 75 ng for hymenoxon and tenulin. The recovery rate of hymenoxon was 102.5 ± 4.5%.
Original language | English (US) |
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Pages (from-to) | 891-895 |
Number of pages | 5 |
Journal | Journal of Agricultural and Food Chemistry |
Volume | 35 |
Issue number | 6 |
DOIs | |
State | Published - Nov 1 1987 |
ASJC Scopus subject areas
- Chemistry(all)
- Agricultural and Biological Sciences(all)