TY - JOUR
T1 - Quantifying the total ionic release from nanoparticles after particle-cell contact
AU - He, Xiao
AU - Pan, Yuanyuan
AU - Zhang, Junzhe
AU - Li, Yuanyuan
AU - Ma, Yuhui
AU - Zhang, Peng
AU - Ding, Yayun
AU - Zhang, Jing
AU - Wu, Zhenqiang
AU - Zhao, Yuliang
AU - Chai, Zhifang
AU - Zhang, Zhiyong
N1 - Funding Information:
This work was financially supported by the Chinese MOST 973 Program ( 2011CB933400 , 2013CB932703 , 2012CB932504 ), and National Natural Science Foundation of China ( 11275215 and 11275218 ).
Publisher Copyright:
© 2014 Elsevier Ltd
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2015/1/1
Y1 - 2015/1/1
N2 - In order to assess the potential hazards of nanoparticles (NPs) releasing, better knowledge about their toxicity to microbes is required. However, it remains controversial whether NPs could exert particle-specific toxicity. In this study, the toxic impacts of four kinds of rare earth oxides (REO) NPs (La2O3, CeO2, Gd2O3, and Yb2O3) on gram-negative Escherichia coli (E. coli) pBR322 were examined. The results indicate that all the tested NPs possessed cytotoxicity against E. coli. To evaluate the ion-related toxicity of REO NPs, the NPs dissolution in the presence of test organisms was quantitatively measured using X-ray absorption fine structure (XAFS) spectroscopy. Our results suggest that NPs-cell contact could facilitate the dissolution of NPs, and the additional ionic release at the particle-cell interface might result in a substantial increase in the ion-related toxicities towards the test organisms. Therefore, the ion-related toxicity of NPs might be grossly underestimated if the additional dissolution of NPs caused by particle-cell contact was overlooked, further leading to a false interpretation of particle-specific toxicity. To the best of our knowledge, this is the first determination of the total NPs dissolution after particle-cell contact. These findings are helpful to advance mechanistic understanding of the toxicity exerted by dissolvable metal-based NPs.
AB - In order to assess the potential hazards of nanoparticles (NPs) releasing, better knowledge about their toxicity to microbes is required. However, it remains controversial whether NPs could exert particle-specific toxicity. In this study, the toxic impacts of four kinds of rare earth oxides (REO) NPs (La2O3, CeO2, Gd2O3, and Yb2O3) on gram-negative Escherichia coli (E. coli) pBR322 were examined. The results indicate that all the tested NPs possessed cytotoxicity against E. coli. To evaluate the ion-related toxicity of REO NPs, the NPs dissolution in the presence of test organisms was quantitatively measured using X-ray absorption fine structure (XAFS) spectroscopy. Our results suggest that NPs-cell contact could facilitate the dissolution of NPs, and the additional ionic release at the particle-cell interface might result in a substantial increase in the ion-related toxicities towards the test organisms. Therefore, the ion-related toxicity of NPs might be grossly underestimated if the additional dissolution of NPs caused by particle-cell contact was overlooked, further leading to a false interpretation of particle-specific toxicity. To the best of our knowledge, this is the first determination of the total NPs dissolution after particle-cell contact. These findings are helpful to advance mechanistic understanding of the toxicity exerted by dissolvable metal-based NPs.
KW - Dissolution
KW - Ion-related toxicity
KW - Particle-cell contact
KW - X-ray absorption fine spectroscopy
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U2 - 10.1016/j.envpol.2014.09.021
DO - 10.1016/j.envpol.2014.09.021
M3 - Article
C2 - 25463714
AN - SCOPUS:84937762082
SN - 0269-7491
VL - 196
SP - 194
EP - 200
JO - Environmental Pollution
JF - Environmental Pollution
ER -