Quantification of substratum contact required for initiation of Colletotrichum graminicola appressoria

Dace Apoga, John Barnard, Harold G. Craighead, Harvey C. Hoch

Research output: Contribution to journalReview articlepeer-review

28 Scopus citations


Colletotrichum graminicola, like many plant pathogenic fungi develop appressoria on germling apices, to facilitate penetration of their host. Induction of these structures occurs after contact with the host surface has been established by the germling. Surface contact and subsequent development of appressoria by germlings of C. graminicola was assessed using interference-reflection microscopy (IRM) and microfabricated pillared silicon substrata. Observations with IRM revealed that under low nutrient conditions, 90% of the germlings developed appressoria once they established 4.5μm of continuous contact with the substratum. Substrata bearing pillars ≤5μm in width supported ≤10% appressoria; however, as pillar width was increased the percentage of appressoria formed increased in a sigmoid fashion to a maximum of 80%. The percentage of appressoria produced experimentally on these surfaces was compared to data sets generated from a model designed to calculate the probability of appressorium development on similar pillar arrays at various germ tube contact lengths. These results indicate that germ tubes of C. graminicola require more than 4μm of continuous contact with a hydrophobic substratum for induction of appressoria.

Original languageEnglish (US)
Pages (from-to)1-12
Number of pages12
JournalFungal Genetics and Biology
Issue number1
StatePublished - Jan 2004


  • Adhesion
  • Appressoria
  • Bovine serum albumin
  • Colletotrichum graminicola
  • Interference-reflection microscopy
  • Microfabrication
  • Pillared surface
  • Surface contact

ASJC Scopus subject areas

  • Genetics
  • Microbiology


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