Quantification of S. aureus adhesion to fibronectin using optical tweezers

Kathryn H. Simpson; M. Gabriela Bowden; Magnus Höök; Bahman Anvari

Quantification of S. aureus adhesion to fibronectin using optical tweezers

Kathryn H. Simpson, M. Gabriela Bowden, Magnus Höök, Bahman Anvari

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Biomaterial infection, a common cause of medical device failure, is initiated by bacterial adhesion to an adsorbed protein layer on the implant surface. This adhesion is thought to be mediated by specific molecules present on the bacterial cell surface. We have used optical tweezers to measure the adhesive force between a single bacterium and a protein-coated surface. A bacterium was optically trapped and brought in contact with a 10-μm diameter polystyrene microsphere coated with fibronectin. The minimum force required to detach the cell from the bead was determined over a range of fibronectin concentrations. The detachment forces were integer multiples of a 25-pN base value that was independent of coating concentration; we propose that the variation in force is related to the number of bonds formed.

Original language	English (US)
Title of host publication	Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Pages	2277-2278
Number of pages	2
Volume	3
State	Published - 2002
Event	Proceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS) - Houston, TX, United States Duration: Oct 23 2002 → Oct 26 2002

Other

Other	Proceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS)
Country	United States
City	Houston, TX
Period	10/23/02 → 10/26/02

Keywords

Bacterial adhesion
Biofilm
Extracellular matrix
Infection

ASJC Scopus subject areas

Bioengineering

Access to Document

Fingerprint Dive into the research topics of 'Quantification of S. aureus adhesion to fibronectin using optical tweezers'. Together they form a unique fingerprint.

Cite this

Simpson, KH, Bowden, MG, Höök, M & Anvari, B 2002, Quantification of S. aureus adhesion to fibronectin using optical tweezers. in Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. vol. 3, pp. 2277-2278, Proceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS), Houston, TX, United States, 10/23/02.

@inproceedings{830eb75a182a4aa1aa42b2cff3f2429f,

title = "Quantification of S. aureus adhesion to fibronectin using optical tweezers",

abstract = "Biomaterial infection, a common cause of medical device failure, is initiated by bacterial adhesion to an adsorbed protein layer on the implant surface. This adhesion is thought to be mediated by specific molecules present on the bacterial cell surface. We have used optical tweezers to measure the adhesive force between a single bacterium and a protein-coated surface. A bacterium was optically trapped and brought in contact with a 10-μm diameter polystyrene microsphere coated with fibronectin. The minimum force required to detach the cell from the bead was determined over a range of fibronectin concentrations. The detachment forces were integer multiples of a 25-pN base value that was independent of coating concentration; we propose that the variation in force is related to the number of bonds formed.",

keywords = "Bacterial adhesion, Biofilm, Extracellular matrix, Infection",

author = "Simpson, {Kathryn H.} and Bowden, {M. Gabriela} and Magnus H{\"o}{\"o}k and Bahman Anvari",

year = "2002",

language = "English (US)",

volume = "3",

pages = "2277--2278",

booktitle = "Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings",

note = "Proceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS) ; Conference date: 23-10-2002 Through 26-10-2002",

}

TY - GEN

T1 - Quantification of S. aureus adhesion to fibronectin using optical tweezers

AU - Simpson, Kathryn H.

AU - Bowden, M. Gabriela

AU - Höök, Magnus

AU - Anvari, Bahman

PY - 2002

Y1 - 2002

N2 - Biomaterial infection, a common cause of medical device failure, is initiated by bacterial adhesion to an adsorbed protein layer on the implant surface. This adhesion is thought to be mediated by specific molecules present on the bacterial cell surface. We have used optical tweezers to measure the adhesive force between a single bacterium and a protein-coated surface. A bacterium was optically trapped and brought in contact with a 10-μm diameter polystyrene microsphere coated with fibronectin. The minimum force required to detach the cell from the bead was determined over a range of fibronectin concentrations. The detachment forces were integer multiples of a 25-pN base value that was independent of coating concentration; we propose that the variation in force is related to the number of bonds formed.

AB - Biomaterial infection, a common cause of medical device failure, is initiated by bacterial adhesion to an adsorbed protein layer on the implant surface. This adhesion is thought to be mediated by specific molecules present on the bacterial cell surface. We have used optical tweezers to measure the adhesive force between a single bacterium and a protein-coated surface. A bacterium was optically trapped and brought in contact with a 10-μm diameter polystyrene microsphere coated with fibronectin. The minimum force required to detach the cell from the bead was determined over a range of fibronectin concentrations. The detachment forces were integer multiples of a 25-pN base value that was independent of coating concentration; we propose that the variation in force is related to the number of bonds formed.

KW - Bacterial adhesion

KW - Biofilm

KW - Extracellular matrix

KW - Infection

UR - http://www.scopus.com/inward/record.url?scp=0036917039&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036917039&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:0036917039

VL - 3

SP - 2277

EP - 2278

BT - Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings

T2 - Proceedings of the 2002 IEEE Engineering in Medicine and Biology 24th Annual Conference and the 2002 Fall Meeting of the Biomedical Engineering Society (BMES / EMBS)

Y2 - 23 October 2002 through 26 October 2002

ER -