Quantification of glycine and taurine conjugated bile acids in human bile using 1H NMR spectroscopy

Omkar B. Ijare, B. S. Somashekar, G. A.Nagana Gowda, Ajay Sharma, V. K. Kapoor, C. L. Khetrapal

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

A simple method for quantification of conjugated bile acids in human bile using 1H NMR spectroscopy is presented. Bile acids in human bile are essentially conjugated with either glycine or taurine. The amide NH resonances from the conjugated bile acids are invariably devoid of interfering signals in 1H NMR spectra. Under physiologic conditions of human bile (pH ∼7.0 to 7.7), amide signal intensities are attenuated due to the chemical exchange and hence quantitative estimation is precluded. In the present study, the quantity of total glycine and taurine conjugated bile acids could be obtained accurately by suppressing the amide exchange by reducing the pH slightly lower than physiologic value (6.0 ± 0.5). Further, the quantity of glycine conjugated bile acids can be calculated accurately by subtracting the quantity of taurine conjugated bile acids from the total conjugated bile acids as determined from the present method.

Original languageEnglish (US)
Pages (from-to)1441-1446
Number of pages6
JournalMagnetic Resonance in Medicine
Volume53
Issue number6
DOIs
StatePublished - Jun 2005

Keywords

  • Glycine and taurine conjugated bile acids
  • H NMR
  • Human bile
  • Quantification

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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