ApoC-II and apo-C-III of human very low density lipoproteins (VLDL) have been quantified by analytical isoelectric focusing (IEF) between pH 4 and 6 in polyacrylamide gels containing 8 M urea. The isoelectric point of apoC-III 0 is pH 4.93; apoC-II, pH 4.78; apoC-III 1, pH 4.72, and apoC-III 2, pH 4.54. ApoC-I is not found in the pH range between pH 4 and 6. Two minor peptides, apoC-IV and apoC-V, with isoelectric points of pH 4.61 and 4.44, respectively, are apoproteins not previously identified. The sensitivity (5-40 μg) and reproducibility (±8%) of this method allow quantitative analysis of apoC-II and apoC-III distribution in VLDL.
|Original language||English (US)|
|Number of pages||6|
|Journal||Journal of lipid research|
|State||Published - 1978|
ASJC Scopus subject areas
- Cell Biology