Abstract

Fluorescence microscopy is a method widely used in life sciences to image biological processes in living and fixed cells or in fixed tissues. Quantification and calibration of images in fluorescence microscopy is notoriously difficult. We have developed a new methodology to prepare tissue " phantoms" that contain known amounts of (i) fluorophore, (ii) DNA, (iii) proteins, and (iv) DNA oligonucleotide standards. The basis of the phantoms is the ability of gelatin to act as a matrix for the conjugation of fluorophores as either a free-flowing liquid or a gelatinous solid depending on temperature (≥40 and ≤4°C).

Original languageEnglish (US)
Pages (from-to)118-126
Number of pages9
JournalAnalytical Biochemistry
Volume404
Issue number2
DOIs
StatePublished - Jan 1 2010

Keywords

  • Blunt-ended
  • Chemotherapy
  • Fluorescence microscopy
  • In situ
  • Ligation
  • Overhanging
  • Phantoms
  • Standards
  • U87

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology

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