TY - JOUR
T1 - Qualitative Alterations of Cytochrome P‐450 in Mouse Liver Microsomes after Administration of Acrylamide and Methylmethacrylate
AU - Nilsen, Odd G.
AU - Toftgård, Rune
AU - Ingelman‐Sundberg, Magnus
AU - Gustafsson, Jan‐Åke
PY - 1978/10
Y1 - 1978/10
N2 - Cytochrome P-450 in mouse liver microsomes was characterized by SDS-polyacrylamide gel electrophoresis after intraperitoneal injection of 80 mg phenobarbital, 4.5 and 45 mg acrylamide and 60 and 600 mg methylmethacrylate per kg body weight each day for four days. Four different forms of cytochrome P-450 with molecular weights of 47,000, 50,000, 54,000 and 56,000 were identified by staining for peroxidase activity and protein. The amount of cytochrome P-450 with a molecular weight of 47,000 (MLvMcP-45047) decreased in the phenobarbital group and in both acrylamide groups. After methylmethacrylate treatment, this form increased at the low dose but was totally repressed at the high dose. The cytochrome P-450 form with a molecular weight of 50,000 (MLvMc P-45050) was significantly increased only in the phenobarbital group. An increase in the total amount of cytochrome P-450 was only observed following treatment with phenobarbital.
AB - Cytochrome P-450 in mouse liver microsomes was characterized by SDS-polyacrylamide gel electrophoresis after intraperitoneal injection of 80 mg phenobarbital, 4.5 and 45 mg acrylamide and 60 and 600 mg methylmethacrylate per kg body weight each day for four days. Four different forms of cytochrome P-450 with molecular weights of 47,000, 50,000, 54,000 and 56,000 were identified by staining for peroxidase activity and protein. The amount of cytochrome P-450 with a molecular weight of 47,000 (MLvMcP-45047) decreased in the phenobarbital group and in both acrylamide groups. After methylmethacrylate treatment, this form increased at the low dose but was totally repressed at the high dose. The cytochrome P-450 form with a molecular weight of 50,000 (MLvMc P-45050) was significantly increased only in the phenobarbital group. An increase in the total amount of cytochrome P-450 was only observed following treatment with phenobarbital.
KW - Cytochrome P‐450
KW - acrylamide
KW - induction
KW - methylmethacrylate
KW - mouse
KW - toxicity
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U2 - 10.1111/j.1600-0773.1978.tb02269.x
DO - 10.1111/j.1600-0773.1978.tb02269.x
M3 - Article
C2 - 716947
AN - SCOPUS:0018128829
SN - 0001-6683
VL - 43
SP - 299
EP - 305
JO - Acta Pharmacologica et Toxicologica
JF - Acta Pharmacologica et Toxicologica
IS - 4
ER -