Qualitative Alterations of Cytochrome P‐450 in Mouse Liver Microsomes after Administration of Acrylamide and Methylmethacrylate

Odd G. Nilsen, Rune Toftgård, Magnus Ingelman‐Sundberg, Jan-Ake Gustafsson

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Cytochrome P-450 in mouse liver microsomes was characterized by SDS-polyacrylamide gel electrophoresis after intraperitoneal injection of 80 mg phenobarbital, 4.5 and 45 mg acrylamide and 60 and 600 mg methylmethacrylate per kg body weight each day for four days. Four different forms of cytochrome P-450 with molecular weights of 47,000, 50,000, 54,000 and 56,000 were identified by staining for peroxidase activity and protein. The amount of cytochrome P-450 with a molecular weight of 47,000 (MLvMcP-45047) decreased in the phenobarbital group and in both acrylamide groups. After methylmethacrylate treatment, this form increased at the low dose but was totally repressed at the high dose. The cytochrome P-450 form with a molecular weight of 50,000 (MLvMc P-45050) was significantly increased only in the phenobarbital group. An increase in the total amount of cytochrome P-450 was only observed following treatment with phenobarbital.

Original languageEnglish (US)
Pages (from-to)299-305
Number of pages7
JournalActa Pharmacologica et Toxicologica
Volume43
Issue number4
DOIs
StatePublished - Jan 1 1978

Keywords

  • Cytochrome P‐450
  • acrylamide
  • induction
  • methylmethacrylate
  • mouse
  • toxicity

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology

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