Qualitative Alterations of Cytochrome P‐450 in Mouse Liver Microsomes after Administration of Acrylamide and Methylmethacrylate

Odd G. Nilsen, Rune Toftgård, Magnus Ingelman‐Sundberg, Jan‐Åke Gustafsson

    Research output: Contribution to journalArticlepeer-review

    9 Scopus citations

    Abstract

    Cytochrome P-450 in mouse liver microsomes was characterized by SDS-polyacrylamide gel electrophoresis after intraperitoneal injection of 80 mg phenobarbital, 4.5 and 45 mg acrylamide and 60 and 600 mg methylmethacrylate per kg body weight each day for four days. Four different forms of cytochrome P-450 with molecular weights of 47,000, 50,000, 54,000 and 56,000 were identified by staining for peroxidase activity and protein. The amount of cytochrome P-450 with a molecular weight of 47,000 (MLvMcP-45047) decreased in the phenobarbital group and in both acrylamide groups. After methylmethacrylate treatment, this form increased at the low dose but was totally repressed at the high dose. The cytochrome P-450 form with a molecular weight of 50,000 (MLvMc P-45050) was significantly increased only in the phenobarbital group. An increase in the total amount of cytochrome P-450 was only observed following treatment with phenobarbital.

    Original languageEnglish (US)
    Pages (from-to)299-305
    Number of pages7
    JournalActa Pharmacologica et Toxicologica
    Volume43
    Issue number4
    DOIs
    StatePublished - Oct 1978

    Keywords

    • Cytochrome P‐450
    • acrylamide
    • induction
    • methylmethacrylate
    • mouse
    • toxicity

    ASJC Scopus subject areas

    • Toxicology
    • Pharmacology

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