TY - JOUR
T1 - PYRIMIDINE SYNTHESIS IN TISSUE CULTURE
AU - Appel, S. H.
AU - Silberberg, D. H.
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1968/12
Y1 - 1968/12
N2 - Abstract— Myelinated cerebellar tissue culture (organ culture) was used to assess the salvage and de novo pathways of pyrimidine synthesis in mammalian brain. Radioactive orotic acid and carbamyl aspartic acid were readily incorporated into UMP and into perchloric acid‐insoluble RNA. The incorporation was effectively blocked by azauridine. Neither radioactive sodium bicarbonate or citrulline was incorporated into UMP or blocked by azauridine. [3H]Uridine, on the other hand, rapidly entered the cultures, was incorporated into UMP and perchloric acid‐insoluble material, and was partially inhibited by azauridine. The failure to demonstrate activity of carbamyl phosphate synthetase suggests the potential importance of the salvage pathway and the likely dependence of the brain upon exogenous and endogenous pyrimidine precursors.
AB - Abstract— Myelinated cerebellar tissue culture (organ culture) was used to assess the salvage and de novo pathways of pyrimidine synthesis in mammalian brain. Radioactive orotic acid and carbamyl aspartic acid were readily incorporated into UMP and into perchloric acid‐insoluble RNA. The incorporation was effectively blocked by azauridine. Neither radioactive sodium bicarbonate or citrulline was incorporated into UMP or blocked by azauridine. [3H]Uridine, on the other hand, rapidly entered the cultures, was incorporated into UMP and perchloric acid‐insoluble material, and was partially inhibited by azauridine. The failure to demonstrate activity of carbamyl phosphate synthetase suggests the potential importance of the salvage pathway and the likely dependence of the brain upon exogenous and endogenous pyrimidine precursors.
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U2 - 10.1111/j.1471-4159.1968.tb05925.x
DO - 10.1111/j.1471-4159.1968.tb05925.x
M3 - Article
C2 - 5729423
AN - SCOPUS:0014393832
SN - 0022-3042
VL - 15
SP - 1437
EP - 1443
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
IS - 12
ER -