Purified glucocorticoid receptors bind selectively in vitro to a cloned DNA fragment that mediates a delayed secondary response to glucocorticoids in vivo

Patrick Hess; T. Meenakshi; Guy Chiu Kai Chan; Jan Carlstedt-Duke; Jan Åke Gustafsson; Farhang Payvar

doi:10.1073/pnas.87.7.2564

Purified glucocorticoid receptors bind selectively in vitro to a cloned DNA fragment that mediates a delayed secondary response to glucocorticoids in vivo

Patrick Hess, T. Meenakshi, Guy Chiu Kai Chan, Jan Carlstedt-Duke, Jan Åke Gustafsson, Farhang Payvar

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

We have identified and characterized a 206-base-pair region downstream from rat α_2u-globulin promoter that specifically mediates a delayed secondary response to glucocorticoids. Unlike positive primary glucocorticoid response elements (GREs), this regulatory element, termed delayed sGRE, dictates an inductive process preceded by a time lag of several hours and blocked by the protein synthesis inhibitor cycloheximide. Reminiscent of GREs and negative GREs (nGREs), a delayed sGRE confers hormonal regulation upon a linked heterologous promoter from a downstream position with respect to transcription start site and, remarkably, also interacts selectively with purified glucocorticoid receptor. These results imply that receptor binding to a delayed sGRE in vivo may mediate certain secondary responses to glucocorticoid hormones.

Original language	English (US)
Pages (from-to)	2564-2568
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	87
Issue number	7
DOIs	https://doi.org/10.1073/pnas.87.7.2564
State	Published - Apr 1990

Keywords

α-globulin gene
Delayed secondary glucocorticoid response element
Late or indirect responses
Specific protein-DNA interactions

ASJC Scopus subject areas

Genetics
General

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10.1073/pnas.87.7.2564

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Hess, P., Meenakshi, T., Chan, G. C. K., Carlstedt-Duke, J., Gustafsson, J. Å., & Payvar, F. (1990). Purified glucocorticoid receptors bind selectively in vitro to a cloned DNA fragment that mediates a delayed secondary response to glucocorticoids in vivo. Proceedings of the National Academy of Sciences of the United States of America, 87(7), 2564-2568. https://doi.org/10.1073/pnas.87.7.2564

Purified glucocorticoid receptors bind selectively in vitro to a cloned DNA fragment that mediates a delayed secondary response to glucocorticoids in vivo. / Hess, Patrick; Meenakshi, T.; Chan, Guy Chiu Kai et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, No. 7, 04.1990, p. 2564-2568.

Research output: Contribution to journal › Article › peer-review

Hess, P, Meenakshi, T, Chan, GCK, Carlstedt-Duke, J, Gustafsson, JÅ & Payvar, F 1990, 'Purified glucocorticoid receptors bind selectively in vitro to a cloned DNA fragment that mediates a delayed secondary response to glucocorticoids in vivo', Proceedings of the National Academy of Sciences of the United States of America, vol. 87, no. 7, pp. 2564-2568. https://doi.org/10.1073/pnas.87.7.2564

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abstract = "We have identified and characterized a 206-base-pair region downstream from rat α2u-globulin promoter that specifically mediates a delayed secondary response to glucocorticoids. Unlike positive primary glucocorticoid response elements (GREs), this regulatory element, termed delayed sGRE, dictates an inductive process preceded by a time lag of several hours and blocked by the protein synthesis inhibitor cycloheximide. Reminiscent of GREs and negative GREs (nGREs), a delayed sGRE confers hormonal regulation upon a linked heterologous promoter from a downstream position with respect to transcription start site and, remarkably, also interacts selectively with purified glucocorticoid receptor. These results imply that receptor binding to a delayed sGRE in vivo may mediate certain secondary responses to glucocorticoid hormones.",

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Purified glucocorticoid receptors bind selectively in vitro to a cloned DNA fragment that mediates a delayed secondary response to glucocorticoids in vivo

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