Purified glucocorticoid receptors bind selectively in vitro to a cloned DNA fragment that mediates a delayed secondary response to glucocorticoids in vivo

Patrick Hess, T. Meenakshi, Guy Chiu Kai Chan, Jan Carlstedt-Duke, Jan Åke Gustafsson, Farhang Payvar

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

We have identified and characterized a 206-base-pair region downstream from rat α2u-globulin promoter that specifically mediates a delayed secondary response to glucocorticoids. Unlike positive primary glucocorticoid response elements (GREs), this regulatory element, termed delayed sGRE, dictates an inductive process preceded by a time lag of several hours and blocked by the protein synthesis inhibitor cycloheximide. Reminiscent of GREs and negative GREs (nGREs), a delayed sGRE confers hormonal regulation upon a linked heterologous promoter from a downstream position with respect to transcription start site and, remarkably, also interacts selectively with purified glucocorticoid receptor. These results imply that receptor binding to a delayed sGRE in vivo may mediate certain secondary responses to glucocorticoid hormones.

Original languageEnglish (US)
Pages (from-to)2564-2568
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number7
DOIs
StatePublished - Apr 1990

Keywords

  • α-globulin gene
  • Delayed secondary glucocorticoid response element
  • Late or indirect responses
  • Specific protein-DNA interactions

ASJC Scopus subject areas

  • Genetics
  • General

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