TY - JOUR
T1 - Purified glucocorticoid receptors bind selectively in vitro to a cloned DNA fragment that mediates a delayed secondary response to glucocorticoids in vivo
AU - Hess, Patrick
AU - Meenakshi, T.
AU - Chan, Guy Chiu Kai
AU - Carlstedt-Duke, Jan
AU - Gustafsson, Jan Åke
AU - Payvar, Farhang
PY - 1990/4
Y1 - 1990/4
N2 - We have identified and characterized a 206-base-pair region downstream from rat α2u-globulin promoter that specifically mediates a delayed secondary response to glucocorticoids. Unlike positive primary glucocorticoid response elements (GREs), this regulatory element, termed delayed sGRE, dictates an inductive process preceded by a time lag of several hours and blocked by the protein synthesis inhibitor cycloheximide. Reminiscent of GREs and negative GREs (nGREs), a delayed sGRE confers hormonal regulation upon a linked heterologous promoter from a downstream position with respect to transcription start site and, remarkably, also interacts selectively with purified glucocorticoid receptor. These results imply that receptor binding to a delayed sGRE in vivo may mediate certain secondary responses to glucocorticoid hormones.
AB - We have identified and characterized a 206-base-pair region downstream from rat α2u-globulin promoter that specifically mediates a delayed secondary response to glucocorticoids. Unlike positive primary glucocorticoid response elements (GREs), this regulatory element, termed delayed sGRE, dictates an inductive process preceded by a time lag of several hours and blocked by the protein synthesis inhibitor cycloheximide. Reminiscent of GREs and negative GREs (nGREs), a delayed sGRE confers hormonal regulation upon a linked heterologous promoter from a downstream position with respect to transcription start site and, remarkably, also interacts selectively with purified glucocorticoid receptor. These results imply that receptor binding to a delayed sGRE in vivo may mediate certain secondary responses to glucocorticoid hormones.
KW - α-globulin gene
KW - Delayed secondary glucocorticoid response element
KW - Late or indirect responses
KW - Specific protein-DNA interactions
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U2 - 10.1073/pnas.87.7.2564
DO - 10.1073/pnas.87.7.2564
M3 - Article
C2 - 1690888
AN - SCOPUS:0025355877
SN - 0027-8424
VL - 87
SP - 2564
EP - 2568
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 7
ER -