Single-stranded DNA (ssDNA) oligonucleotides are useful as aptamers, hybridization probes and for emerging applications in DNA nanotechnology. Current methods to purify ssDNA require both a strand-separation step and a separate size-separation step but may still leave double-stranded DNA (dsDNA) impurities in the sample. Here, we use commercially available acrydite DNA primers to immobilize one strand of a PCR product within a polyacrylamide matrix. Electrophoresis moves the non-crosslinked DNA into the gel where the single-stranded product of desired size can be recovered. Our results show this method produces high yields of pure ssDNA.
- Aptamers, Nucleotide/chemistry
- DNA Primers/chemistry
- DNA, Single-Stranded/chemistry
- Electrophoresis, Polyacrylamide Gel/instrumentation
- Equipment Design
- Immobilized Nucleic Acids/chemistry