Abstract
Single-stranded DNA (ssDNA) oligonucleotides are useful as aptamers, hybridization probes and for emerging applications in DNA nanotechnology. Current methods to purify ssDNA require both a strand-separation step and a separate size-separation step but may still leave double-stranded DNA (dsDNA) impurities in the sample. Here, we use commercially available acrydite DNA primers to immobilize one strand of a PCR product within a polyacrylamide matrix. Electrophoresis moves the non-crosslinked DNA into the gel where the single-stranded product of desired size can be recovered. Our results show this method produces high yields of pure ssDNA.
Original language | English (US) |
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Pages (from-to) | 275-282 |
Number of pages | 8 |
Journal | BioTechniques |
Volume | 62 |
Issue number | 6 |
DOIs | |
State | Published - Jun 1 2017 |
Keywords
- Acrylamide/chemistry
- Aptamers, Nucleotide/chemistry
- DNA Primers/chemistry
- DNA, Single-Stranded/chemistry
- Electrophoresis, Polyacrylamide Gel/instrumentation
- Equipment Design
- Immobilized Nucleic Acids/chemistry
- Polymerization