TY - JOUR
T1 - Purification and distribution of a major protein in rat prostate that binds estramustine, a nitrogen mustard derivative of estradiol-17β
AU - Forsgren, B.
AU - Bjork, P.
AU - Carlstrom, K.
AU - Gustafsson, J. A.
AU - Pousette, A.
AU - Högberg, B.
PY - 1979
Y1 - 1979
N2 - A protein in rat ventral prostate cytosol that binds estramustine [estradiol 3-bis(2-chloroethyl)carbamate] was purified to homogeneity by using chromatography on DEAE-cellulose, Sephadex G-100 (superfine), octyl-Sepharose CL-4B, and polyacrylamide gel electrophoresis. The estramustine-binding protein was found to have a M(r) of 46,000 as estimated by gel filtration. After analysis by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate, the protein was found to consist of two subunits with M(r) of about 20,000 and 18,000. After reduction of disulfide bridges, the protein was decomposed into three components with M(r) of about 12,000, 11,000, and 8000. Antibodies against the protein were raised in rabbits and a radioimmunoassay was developed for it. The estramustine-binding protein constituted about 18% of the total protein in rat ventral prostate cytosol, was present in the dorsal and lateral lobes of the prostate, and was also detected in the pituitary gland, cerebral cortex, submaxillary gland, thyroid gland, adrenal gland, seminal vesicle, coagulating gland, epididymis, and preputial gland of the male rat. In female rats the protein was detected in cerebral cortex. Because the estramustine-binding protein is predominantly found in the accessory sexual glands of the male rat, it may be of importance for maintaining male fertility.
AB - A protein in rat ventral prostate cytosol that binds estramustine [estradiol 3-bis(2-chloroethyl)carbamate] was purified to homogeneity by using chromatography on DEAE-cellulose, Sephadex G-100 (superfine), octyl-Sepharose CL-4B, and polyacrylamide gel electrophoresis. The estramustine-binding protein was found to have a M(r) of 46,000 as estimated by gel filtration. After analysis by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate, the protein was found to consist of two subunits with M(r) of about 20,000 and 18,000. After reduction of disulfide bridges, the protein was decomposed into three components with M(r) of about 12,000, 11,000, and 8000. Antibodies against the protein were raised in rabbits and a radioimmunoassay was developed for it. The estramustine-binding protein constituted about 18% of the total protein in rat ventral prostate cytosol, was present in the dorsal and lateral lobes of the prostate, and was also detected in the pituitary gland, cerebral cortex, submaxillary gland, thyroid gland, adrenal gland, seminal vesicle, coagulating gland, epididymis, and preputial gland of the male rat. In female rats the protein was detected in cerebral cortex. Because the estramustine-binding protein is predominantly found in the accessory sexual glands of the male rat, it may be of importance for maintaining male fertility.
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U2 - 10.1073/pnas.76.7.3149
DO - 10.1073/pnas.76.7.3149
M3 - Article
C2 - 290993
AN - SCOPUS:0018743155
VL - 76
SP - 3149
EP - 3153
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 7
ER -