Purification and Characterization of a Central Cholinergic Enhancing Factor from Rat Brain: Its Identity as Phosphoethanolamine

J. Robert Bostwick, Diane W. Landers, Garrett Crawford, Kenneth Lau, Stanley H. Appel

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

A compound that can enhance the apparent synthesis of acetylcholine in cultured explants in the medial septal nucleus has been purified from rat brain and identified as phosphoethanolamine. Acetylcholine synthesis is stimulated two- to threefold in cultures grown for 5 days in the presence of phosphoethanolamine, ethanolamine, or cytidine 5'-diphosphoethanolamine at concentrations above 100 μM. This effect appears to result from an increase in the accumulation of choline via the high-affinity, sodium-dependent uptake mechanism. The development of choline acetyltransferase activity is not affected. Phosphoethanolamine and ethanolamine seem to enhance the ability of developing cholinergic neurons to utilize choline accumulated via the sodium-dependent high-affinity choline uptake mechanism for the preferential production of acetylcholine without increasing the general metabolism of the cultures. Choline itself and its related derivatives are not stimulatory for these effects.

Original languageEnglish (US)
Pages (from-to)448-458
Number of pages11
JournalJournal of Neurochemistry
Volume53
Issue number2
DOIs
StatePublished - Jan 1 1989

Keywords

  • Acetylcholine synthesis
  • Phosphoethanolamine
  • Septal nucleus
  • Tissue culture

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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